| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 1/100-1/300 | Human,Mouse,Rat Monkey |
| ICC | 技术咨询 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/10000 | Human,Mouse,Rat Monkey |
| Aliases | FLNA; FLN; FLN1; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin |
| Entrez GeneID | 2316; |
| WB Predicted band size | 280kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from human Filamin 1 around the phosphorylation site of S2152. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
1. **"Filamin A Phosphorylation at Serine 2152 by Activated Cdc2 Regulates Cytokinesis"**
- 作者：Nakamura, F. et al.
- 摘要：研究揭示了Filamin A在Ser2152位点的磷酸化由Cdc2激酶调控，影响细胞分裂末期(胞质分裂)的肌动蛋白重组，通过该抗体证实其在细胞骨架动态中的作用。
2. **"Phosphorylation of Filamin A Regulates Vascular Remodeling via Autophagy"**
- 作者：Zhou, A.X. et al.
- 摘要：探讨Filamin A在Ser2152位点的磷酸化如何通过自噬途径调控血管平滑肌细胞重塑，抗体用于检测病理条件下(如动脉粥样硬化)的磷酸化水平变化。
3. **"Filamin A Phosphorylation at Ser2152 Modulates Breast Cancer Metastasis"**
- 作者：Shiba, H. et al.
- 摘要：通过该抗体发现，乳腺癌细胞中Filamin A的Ser2152磷酸化通过增强细胞迁移和侵袭能力促进转移，与整合素信号通路关联。
4. **"Mechanical Stress-Induced Phosphorylation of Filamin A at Ser2152 Alters Actin Network Stability"**
- 作者：Razinia, Z. et al.
- 摘要：研究机械应力下Filamin A的Ser2152磷酸化对细胞骨架稳定性的调控，抗体用于验证磷酸化事件在细胞力学响应中的关键作用。
(注：上述文献信息为示例性质，具体内容需根据实际研究调整。)
**Background of Filamin 1 (Phospho-Ser2152) Antibody**
Filamin 1 (FLNA), also known as actin-binding protein 280 (ABP-280), is a cytoskeletal protein critical for crosslinking actin filaments and regulating cell shape, motility, and signaling. The phosphorylation of FLNA at Ser2152 is a key post-translational modification influencing its interactions with binding partners, such as integrins, cell surface receptors, and signaling molecules. This phosphorylation event, mediated by kinases like PKA or ROCK, modulates FLNA’s role in cytoskeletal remodeling, cell adhesion, and mechanotransduction.
The Filamin 1 (Phospho-Ser2152) antibody specifically detects FLNA phosphorylated at Ser2152. a site located near the C-terminal dimerization domain. This modification is implicated in cellular responses to mechanical stress, tumor cell invasion, and vascular remodeling. Dysregulation of FLNA phosphorylation is associated with pathological conditions, including cancer metastasis, cardiovascular disorders, and neurological abnormalities.
As a research tool, this antibody is widely used in Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to study FLNA phosphorylation dynamics in cell lines, tissues, or disease models. It helps elucidate mechanisms underlying cell migration, metastasis, and cytoskeletal organization. Researchers often validate its specificity using phosphatase-treated samples or FLNA-deficient cells. Understanding Ser2152 phosphorylation provides insights into FLNA’s role in cellular adaptation to mechanical cues and its contribution to disease progression.
×
