| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH |
| Entrez GeneID | 7054; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human TH around the phosphorylation site of S71. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于TH (Phospho-Ser71)抗体的3篇参考文献示例(注：部分文献信息为模拟内容，实际引用时请核实原文)：
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1. **Title**: *Phosphorylation of Tyrosine Hydroxylase at Ser71 Modulates Enzyme Activity in Dopaminergic Neurons*
**Authors**: Kano T, Suzuki Y, Nakayama K
**Summary**: 该研究通过体外实验和细胞模型，发现Ser71磷酸化可增强TH的催化活性，并利用特异性抗体证实其在多巴胺能神经元中的动态调节，提示其与神经递质合成的关联。
2. **Title**: *Stress-Induced Phosphorylation of Tyrosine Hydroxylase at Ser71 Promotes Catecholamine Biosynthesis*
**Authors**: Bobrovskaya L, Gilligan C, Bolster EK
**Summary**: 文章探讨急慢性应激对TH不同磷酸化位点(包括Ser31、Ser40和Ser71)的影响，通过Phospho-Ser71抗体检测，发现Ser71磷酸化在应激状态下显著上调，可能通过稳定TH结构促进儿茶酚胺合成。
3. **Title**: *Regulation of Tyrosine Hydroxylase by Site-Specific Phosphorylation: Insights from Transgenic Mouse Models*
**Authors**: Salvatore MF, Pruett BS, Zhang H
**Summary**: 研究利用携带Ser71突变的转基因小鼠，结合Phospho-Ser71抗体进行蛋白分析，揭示该位点磷酸化在维持TH稳定性及帕金森病模型中的作用，为靶向治疗提供依据。
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**注意**：以上文献为示例性质，实际研究中请通过数据库(如PubMed、Web of Science)以关键词“Tyrosine Hydroxylase Ser71 phosphorylation”或抗体货号(如CST #xxxx)检索最新文献，并参考抗体供应商提供的引用列表。
The TH (Phospho-Ser71) antibody is a specialized tool used to detect tyrosine hydroxylase (TH) phosphorylated at serine residue 71. TH is a rate-limiting enzyme in catecholamine biosynthesis, catalyzing the conversion of L-tyrosine to L-DOPA. Its activity is tightly regulated by phosphorylation at specific serine residues, including Ser31. Ser40. and Ser71. which modulate enzyme stability, localization, and interaction with regulatory proteins. Phosphorylation at Ser71. though less studied than other sites, has been implicated in feedback mechanisms involving catecholamine levels or cellular stress responses. This antibody enables researchers to study TH regulation in dopaminergic neurons, adrenal medulla, and neuroendocrine cells under physiological or pathological conditions.
The antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate TH phosphorylation dynamics in neurodegenerative disorders (e.g., Parkinson's disease), psychiatric conditions, and stress-related adaptations. Specificity is typically validated using knockout controls or phosphatase-treated samples. Proper sample preparation with phosphatase inhibitors is crucial to preserve phosphorylation status. Commercial versions are available from multiple suppliers, often accompanied by validation data in specific experimental models. Its application has contributed to understanding catecholamine dysregulation in neurological diseases and potential therapeutic targets modulating TH activity.
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