| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | LIPE; Hormone-sensitive lipase; HSL |
| Entrez GeneID | 3991; |
| WB Predicted band size | 83kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human HSL around the phosphorylation site of S855. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于HSL (Phospho-Ser855)抗体的参考文献示例(部分内容为模拟，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *Adrenergic Regulation of Lipolysis in Adipocytes: Phosphorylation of Hormone-Sensitive Lipase at Ser855*
**作者**: Smith A, et al.
**摘要**: 本研究利用Phospho-Ser855特异性抗体，揭示了β-肾上腺素能受体激活后通过PKA途径磷酸化HSL的Ser855位点，促进脂肪分解的分子机制。抗体验证显示其对Ser855磷酸化具有高特异性，并通过基因敲除实验证实了该位点的功能。
2. **文献名称**: *Role of HSL Phosphorylation in Insulin Resistance: Insights from Ser855 Mutant Models*
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫沉淀技术，使用Phospho-Ser855抗体分析了肥胖小鼠模型中HSL的磷酸化水平变化，发现胰岛素抵抗状态下Ser855磷酸化显著降低，提示其与代谢调控的关联。
3. **文献名称**: *Development and Validation of a Novel Phospho-Specific Antibody for HSL Ser855*
**作者**: Johnson R, et al.
**摘要**: 报道了一种针对HSL Ser855磷酸化位点的多克隆抗体的开发过程，通过肽段免疫和亲和纯化获得高灵敏度抗体。实验表明该抗体适用于免疫荧光和流式细胞术，为脂肪代谢研究提供新工具。
4. **文献名称**: *Cross-Talk Between HSL Phosphorylation Sites: Ser855 and Ser563 in Lipolytic Activation*
**作者**: Tanaka K, et al.
**摘要**: 利用Phospho-Ser855抗体与Ser563抗体对比，发现两种磷酸化位点在脂肪分解中的协同作用。研究证实Ser855磷酸化可能通过构象变化增强HSL的酶活性。
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**注意**：以上文献信息为示例，实际引用时需通过PubMed、Google Scholar等平台检索真实文献(关键词：HSL, Phospho-Ser855. hormone-sensitive lipase, phosphorylation)。若文献较少，可扩展至HSL其他磷酸化位点(如Ser563)或相关代谢通路研究。
The HSL (Phospho-Ser855) antibody is a specialized tool used to study the phosphorylation state of hormone-sensitive lipase (HSL), a key enzyme regulating lipid metabolism. HSL hydrolyzes stored triglycerides in adipose tissue and other organs, releasing free fatty acids for energy production. Its activity is tightly controlled by phosphorylation at specific serine residues, including Ser855. which is critical for enzyme activation and translocation to lipid droplets. Phosphorylation at Ser855 is primarily mediated by cyclic AMP (cAMP)-dependent protein kinase A (PKA) in response to hormonal signals like adrenaline and noradrenaline, promoting lipolysis during energy-demanding states.
This antibody specifically detects HSL when phosphorylated at Ser855. enabling researchers to investigate dynamic post-translational modifications of HSL under various physiological or pathological conditions, such as obesity, diabetes, or metabolic syndrome. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess HSL activation status in cell or tissue samples. Validated for specificity in multiple species, including humans and rodents, the antibody aids in elucidating mechanisms of lipid homeostasis, insulin resistance, and catecholamine signaling. Its application contributes to understanding how dysregulated HSL phosphorylation impacts metabolic diseases, potentially guiding therapeutic strategies targeting lipolytic pathways.
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