| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LYN; JTK8; Tyrosine-protein kinase Lyn; Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn |
| Entrez GeneID | 4067; |
| WB Predicted band size | 58kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Lyn around the phosphorylation site of Y508. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Lyn (Phospho-Tyr508)抗体的3篇参考文献示例(注：以下内容为示例，实际文献可能需要根据具体研究补充或调整)：
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1. **文献名称**: *"Regulation of Src-family kinase activity by C-terminal phosphorylation in chronic lymphocytic leukemia"*
**作者**: Hibbs ML, et al.
**摘要**: 本研究利用Phospho-Tyr508特异性抗体，揭示了Lyn激酶在慢性淋巴细胞白血病(CLL)中的异常激活机制。研究发现，Tyr508位点的磷酸化负向调控Lyn活性，而CLL患者中该位点的低磷酸化状态导致Lyn持续活化，促进癌细胞存活信号通路。
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2. **文献名称**: *"Phosphorylation of Lyn tyrosine kinase at Tyr508 modulates B-cell receptor signaling outcomes"*
**作者**: Lowell CA, et al.
**摘要**: 通过Phospho-Tyr508抗体检测，作者证明B细胞受体(BCR)激活后，Lyn的Tyr508磷酸化水平升高，进而抑制其激酶活性。该调控机制在维持B细胞耐受性和防止自身免疫反应中起关键作用。
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3. **文献名称**: *"Targeted disruption of the Lyn tyrosine kinase gene reveals its role in FcεRI-dependent mast cell activation"*
**作者**: Nishizumi H, et al.
**摘要**: 研究使用Phospho-Tyr508抗体分析肥大细胞中Lyn的激活状态，发现FcεRI受体介导的过敏反应中，Tyr508磷酸化水平动态变化，影响Lyn对下游分子(如Syk激酶)的招募及脱颗粒信号传递。
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**注**：以上文献为示例，实际引用时请通过PubMed、Google Scholar等平台核实具体信息，并确保磷酸化位点编号与目标物种(人/小鼠)一致(如人Lyn Tyr508对应小鼠Lyn Tyr507)。
Lyn (Phospho-Tyr508) antibody is a specialized tool used to detect the phosphorylated form of the Lyn kinase at tyrosine residue 508. Lyn, a member of the Src family of tyrosine kinases, plays a critical role in signal transduction within immune cells, particularly B cells and myeloid cells. It regulates diverse processes, including cell proliferation, differentiation, and immune responses. Phosphorylation at Tyr508 (located in the C-terminal regulatory domain) is a key post-translational modification associated with Lyn's activity. This site corresponds to a conserved inhibitory phosphorylation motif in Src-family kinases. When phosphorylated, Tyr508 promotes intramolecular interactions that stabilize the kinase in an inactive conformation, thereby downregulating its enzymatic activity.
The Lyn (Phospho-Tyr508) antibody is widely used in research to study the activation status and regulatory mechanisms of Lyn in physiological and pathological contexts. It helps investigate signaling pathways involving B-cell receptors, Fc receptors, and cytokine receptors, as well as aberrant Lyn activation in diseases like cancer and autoimmune disorders. This antibody is typically validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry, often in samples from humans, mice, or rats. Its specificity for the phosphorylated form allows researchers to distinguish active versus inactive Lyn states, providing insights into cellular signaling dynamics and therapeutic targeting.
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