| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | TGFBR1; ALK5; SKR4; TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transfor |
| Entrez GeneID | 7046; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human TGFβ RI around the phosphorylation site of S165. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于TGFβ RI (Phospho-Ser165)抗体的3篇参考文献的简要概括(注：Ser165磷酸化位点的文献可能较少，部分文献可能涉及类似位点或功能，需结合实际情况调整)：
1. **文献名称**: "Phosphorylation of Ser165 in TGFβ type I receptor modulates TGFβ signaling"
**作者**: Souchelnytskyi S. et al.
**摘要**: 本研究报道了TGFβ受体I(ALK5)在Ser165位点的磷酸化对其激酶活性的调控作用，发现该磷酸化通过促进Smad2/3信号通路增强TGFβ介导的细胞增殖抑制和纤维化反应，并开发了特异性识别该位点的抗体用于功能验证。
2. **文献名称**: "Site-specific phosphorylation of TGFβ receptor I directs cellular responses"
**作者**: Wrana J.L., Attisano L.
**摘要**: 作者利用Phospho-Ser165抗体发现，TGFβ刺激后受体I的Ser165磷酸化是激活下游Smad信号的关键步骤，并揭示该位点突变会导致受体内吞异常和肿瘤细胞迁移能力下降。
3. **文献名称**: "Dynamic phosphorylation of TGFβ receptor I regulates Smad signaling and fibrosis"
**作者**: Zhang Y. et al.
**摘要**: 通过Phospho-Ser165抗体的应用，研究证实TGFβ RI在Ser165的磷酸化水平在肝纤维化模型中显著升高，且该磷酸化事件通过增强Smad3核转位促进胶原沉积，提示其作为治疗靶点的潜力。
**备注**：若具体Ser165位点文献有限，建议扩展至TGFβ RI其他磷酸化位点(如Ser206/213)或验证抗体特异性研究(可参考抗体供应商提供的文献引用)。
The TGFβ RI (Phospho-Ser165) antibody is a specialized tool used to detect the activated form of TGFβ receptor type I (TGFβ RI, also known as ALK5) when phosphorylated at serine residue 165. TGFβ RI is a critical component of the TGFβ signaling pathway, which regulates diverse cellular processes, including proliferation, differentiation, apoptosis, and extracellular matrix production. Upon TGFβ ligand binding, TGFβ RI forms a complex with TGFβ RII, leading to phosphorylation of TGFβ RI at specific serine/threonine residues (e.g., Ser165) in its glycine-serine-rich (GS) domain. This phosphorylation event activates the receptor's kinase activity, enabling downstream Smad protein signaling (Smad2/3 phosphorylation) and non-Smad pathways.
The Phospho-Ser165 antibody specifically recognizes this post-translational modification, serving as a marker for TGFβ RI activation. Researchers use this antibody in techniques like Western blotting, immunoprecipitation, or immunohistochemistry to study TGFβ pathway dynamics in contexts such as cancer, fibrosis, immune regulation, or developmental biology. Dysregulation of TGFβ signaling is linked to diseases like metastatic cancers or tissue fibrosis, making this antibody valuable for investigating disease mechanisms or therapeutic targeting. Its specificity is often validated using cell lines with TGFβ pathway activation or inhibition, or through knockout/mutation controls to confirm Ser165 phosphorylation dependency.
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