| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | TIRAP; MAL; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein |
| Entrez GeneID | 114609; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human TIRAP around the phosphorylation site of Y86. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **TIRAP (Phospho-Tyr86) 抗体** 的模拟参考文献示例(仅供参考，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"TIRAP Phosphorylation at Tyr86 Is Critical for TLR4-Mediated NF-κB Activation"*
**作者**: Smith A, et al.
**摘要**: 研究证实 TIRAP 在 TLR4 信号通路中 Tyr86 位点的磷酸化是招募下游分子 MyD88 的关键步骤。通过使用 Phospho-Tyr86 特异性抗体进行免疫沉淀和 Western blot，作者发现该磷酸化事件在 LPS 诱导的巨噬细胞炎症反应中起核心作用。
2. **文献名称**: *"Development and Application of a Phospho-Specific Antibody for TIRAP Tyr86 in Innate Immunity Studies"*
**作者**: Zhang L, et al.
**摘要**: 本文报道了一种针对 TIRAP Tyr86 磷酸化位点的多克隆抗体的开发，验证了其在免疫荧光和流式细胞术中的特异性。实验表明，该抗体能有效检测细菌感染后 TIRAP 的激活状态，为先天免疫研究提供工具。
3. **文献名称**: *"Phosphorylation-Dependent Regulation of TIRAP Subcellular Localization by Src Kinases"*
**作者**: Johnson R, et al.
**摘要**: 研究揭示 Src 激酶介导 TIRAP Tyr86 磷酸化，并利用 Phospho-Tyr86 抗体观察其从细胞质向细胞膜的转位。该磷酸化事件是 TLR2/4 信号启动的必要条件，缺失突变体会显著抑制促炎细胞因子的产生。
4. **文献名称**: *"TIRAP Tyrosine 86 Phosphorylation as a Biomarker in Sepsis-Induced Inflammation"*
**作者**: Brown K, et al.
**摘要**: 通过 Phospho-Tyr86 抗体检测脓毒症患者外周血单核细胞中 TIRAP 的磷酸化水平，发现其与疾病严重程度正相关。研究提示该抗体可作为评估过度炎症反应的潜在标志物。
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**注意**：以上为模拟生成的参考文献，实际研究中请通过 **PubMed、Web of Science 或 Google Scholar** 等平台检索真实文献，并核实抗体应用的具体实验条件及结论。
The TIRAP (Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein), also known as Mal, is a critical adaptor molecule in innate immune signaling. It mediates downstream signaling of Toll-like receptors (TLRs) 2 and 4 by bridging activated TLRs to the MyD88-dependent pathway, ultimately triggering NF-κB activation and pro-inflammatory cytokine production. Phosphorylation at Tyr86 is a key post-translational modification regulating TIRAP activity. This phosphorylation event, likely mediated by Src family kinases, facilitates conformational changes that enhance TIRAP's interaction with downstream signaling components, such as MyD88. thereby amplifying TLR-driven immune responses.
The TIRAP (Phospho-Tyr86) antibody specifically detects TIRAP phosphorylated at tyrosine 86. serving as a vital tool for studying TLR signaling dynamics. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess TIRAP activation status in response to pathogens, inflammatory stimuli, or therapeutic agents. Its specificity is often validated using knockout controls or phospho-blocking peptides.
Studies employing this antibody have elucidated TIRAP's role in infectious diseases, sepsis, and autoimmune disorders, highlighting its potential as a therapeutic target. Dysregulation of TIRAP phosphorylation has been linked to chronic inflammation and immune evasion mechanisms, making this antibody valuable for both basic research and drug development.
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