| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | S1PR1; CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD antigen CD363 |
| Entrez GeneID | 1901; |
| WB Predicted band size | 42kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human EDG-1 around the phosphorylation site of T236. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于EDG-1 (Phospho-Thr236)抗体的3篇参考文献(基于模拟生成内容，非真实文献)：
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1. **文献名称**: *"Phosphorylation of EDG-1 at Thr236 Regulates S1P-Mediated Endothelial Cell Migration"*
**作者**: Lee et al.
**摘要**: 研究揭示了EDG-1(S1PR1)在Thr236位点的磷酸化通过调控Rho GTPase活性影响内皮细胞迁移。实验中采用Phospho-Thr236特异性抗体进行Western blot和免疫荧光分析，证实该位点磷酸化在S1P信号通路中的关键作用。
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2. **文献名称**: *"EDG-1 Thr236 Phosphorylation Modulates Immune Cell Trafficking in Inflammatory Disease"*
**作者**: Smith et al.
**摘要**: 本文利用EDG-1 (Phospho-Thr236)抗体在小鼠模型中检测磷酸化水平，发现Thr236磷酸化增强S1PR1内吞作用，从而影响淋巴细胞归巢。研究为炎症性疾病治疗提供了潜在靶点。
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3. **文献名称**: *"Antibody-Based Detection of EDG-1 Phospho-Thr236 in Cancer Metastasis"*
**作者**: Zhang et al.
**摘要**: 通过开发高特异性EDG-1 (Phospho-Thr236)抗体，研究证明该位点磷酸化与乳腺癌细胞转移能力正相关。抗体用于组织芯片分析，显示磷酸化水平与患者预后显著相关。
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注：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“S1PR1 Phospho-Thr236 antibody”或“EDG-1 Thr236 phosphorylation”检索最新文献。
The EDG-1 (Phospho-Thr236) antibody is designed to detect the phosphorylated form of EDG-1 (Endothelial Differentiation Gene-1), also known as S1PR1 (Sphingosine-1-Phosphate Receptor 1), at threonine residue 236. EDG-1 is a G protein-coupled receptor that binds sphingosine-1-phosphate (S1P), a bioactive lipid regulating diverse cellular processes, including cell migration, angiogenesis, immune response, and vascular development. Phosphorylation of Thr236 in EDG-1 is a critical post-translational modification associated with receptor desensitization and internalization, a regulatory mechanism to attenuate S1P signaling. This phosphorylation event is often mediated by G protein-coupled receptor kinases (GRKs) following receptor activation.
The EDG-1 (Phospho-Thr236) antibody is widely used in research to study S1PR1 signaling dynamics, receptor trafficking, and downstream pathways in physiological or pathological contexts, such as inflammation, cancer, or cardiovascular diseases. It is validated for applications like Western blotting, immunohistochemistry, and immunofluorescence, enabling the detection of activated EDG-1 in cell lines or tissue samples. By targeting the phosphorylated state, this antibody helps elucidate the spatiotemporal regulation of S1PR1 activity and its role in disease mechanisms. Researchers often utilize it alongside total EDG-1 antibodies to assess phosphorylation levels relative to total receptor expression, providing insights into signaling modulation. Proper controls, including phosphorylation-inducing treatments (e.g., S1P stimulation) or kinase inhibitors, are recommended to ensure specificity.
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