| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | ANXA1; ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35 |
| Entrez GeneID | 301; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from human Annexin I around the phosphorylation site of Y21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 Annexin I (Phospho-Tyr21) 抗体的参考文献示例(内容为虚构，仅供格式参考)：
1. **"Tyrosine phosphorylation of Annexin A1 regulates membrane binding and cellular localization"**
- Author: Smith J, et al.
- 摘要：研究揭示了 Annexin I 在 Tyr21 位点的磷酸化如何通过调控其与细胞膜的相互作用影响胞内定位，并利用特异性抗体验证了磷酸化依赖的膜解离现象。
2. **"Phosphorylation-dependent signaling of Annexin A1 in inflammation"**
- Author: Lee H, et al.
- 摘要：通过免疫印迹和免疫荧光技术，证明 Tyr21 磷酸化修饰的 Annexin I 参与炎症反应中 NF-κB 通路的负向调控，相关抗体被用于检测巨噬细胞模型中的修饰水平。
3. **"Annexin A1 phosphorylation at Tyr21 promotes cancer cell migration"**
- Author: Wang X, et al.
- 摘要：利用 Phospho-Tyr21 特异性抗体，研究发现乳腺癌细胞中 Annexin I 的磷酸化通过激活 ERK 通路增强细胞迁移能力，提示其作为潜在治疗靶点。
4. **"Development of a monoclonal antibody targeting Annexin A1 phosphorylated at tyrosine 21"**
- Author: Garcia R, et al.
- 摘要：文献描述了该抗体的制备与验证，包括在体外激酶实验和病理组织中的特异性检测，证实其在研究 Annexin I 磷酸化功能中的可靠性。
**注**：以上文献为示例，实际文献需通过 PubMed 或 Google Scholar 以关键词 "Annexin A1 Phospho-Tyr21" 或 "ANXA1 pTyr21 antibody" 检索。
Annexin I (AnxA1) is a calcium-dependent phospholipid-binding protein belonging to the annexin superfamily, involved in membrane trafficking, anti-inflammatory responses, and cell signaling. It consists of a conserved C-terminal core domain mediating membrane interactions and a unique N-terminal domain responsible for regulatory functions. Phosphorylation at Tyr21 (Y21) in the N-terminal domain modulates AnxA1 activity by altering its conformation and interactions with binding partners, such as S100A11. thereby influencing membrane aggregation, exocytosis, and extracellular signaling. This post-translational modification is often mediated by tyrosine kinases like Src family kinases, linking AnxA1 to pathways regulating cell proliferation, apoptosis, and immune regulation.
The Annexin I (Phospho-Tyr21) antibody specifically detects Y21-phosphorylated AnxA1. enabling researchers to study its activation status in cellular processes. Dysregulation of AnxA1 phosphorylation has been implicated in inflammatory diseases, cancer progression, and autoimmune disorders. For example, elevated Tyr21 phosphorylation may enhance AnxA1’s anti-inflammatory effects by promoting its interaction with formyl peptide receptors, while in cancer, it can either suppress or promote metastasis depending on context. This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore AnxA1’s role in phosphorylation-dependent signaling networks, offering insights into therapeutic targeting of related pathways.
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