| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tyrosine-protein kinase JAK2/JAK3 (EC 2.7.10.2) (Janus kinase 2/Janus kinase 3) (JAK-2/JAK-3) |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized Phospho peptide derived from human JAK2/3.at AA range: T966 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于JAK2/3 (Phospho-Tyr966/939)抗体的参考文献示例(注：部分内容为合理虚构，建议根据实际文献调整)：
1. **"JAK2 Tyrosine 966 Phosphorylation Regulates Kinase Activity in Erythropoietin Signaling"**
- **作者**: Smith A, et al.
- **摘要**: 研究利用Phospho-Tyr966抗体揭示JAK2在促红细胞生成素受体激活后的自磷酸化机制，证明Tyr966磷酸化增强JAK2激酶活性，并促进STAT5信号传导。
2. **"JAK3 Phosphorylation at Tyrosine 939 Mediates IL-2 Receptor Signaling in T Cells"**
- **作者**: Lee B, et al.
- **摘要**: 通过Phospho-Tyr939抗体检测发现，JAK3在IL-2刺激下于Tyr939位点磷酸化，调控下游PI3K/AKT通路，影响T细胞增殖与存活。
3. **"Comparative Analysis of JAK2 and JAK3 Activation in Myeloproliferative Disorders"**
- **作者**: Garcia R, et al.
- **摘要**: 采用Phospho-Tyr966/939抗体对比JAK2和JAK3在骨髓增殖性疾病中的激活差异，发现JAK2突变体磷酸化水平与疾病严重程度相关。
4. **"Structural Insights into JAK3 Autoinhibition and Phosphorylation-Dependent Activation"**
- **作者**: Chen X, et al.
- **摘要**: 结合晶体学与Phospho-Tyr939抗体实验，阐明JAK3自抑制构象及Tyr939磷酸化对其激酶结构域开放的调控机制。
**建议**：实际引用时可通过PubMed或抗体供应商(如CST、Abcam)提供的文献列表检索具体研究，关键词包括“JAK2 Tyr966 phosphorylation”或“JAK3 Tyr939 antibody”。
The JAK2/3 (Phospho-Tyr966/939) antibody is a crucial tool for studying the activation and signaling mechanisms of Janus kinase 2 (JAK2) and JAK3. members of the JAK tyrosine kinase family. These kinases play pivotal roles in cytokine receptor-mediated signaling, particularly through the JAK-STAT pathway, which regulates immune responses, cell proliferation, and hematopoiesis. Phosphorylation at specific tyrosine residues (Tyr966 in JAK2 and Tyr939 in JAK3) is associated with kinase activation. Upon cytokine binding to receptors, JAKs autophosphorylate these sites, enabling downstream STAT protein recruitment and signaling. Dysregulation of JAK2/3 phosphorylation is linked to pathologies, including myeloproliferative disorders, autoimmune diseases, and cancers.
This antibody specifically detects phosphorylated Tyr966 (JAK2) and Tyr939 (JAK3), facilitating research into JAK activation states. It is widely used in techniques like Western blotting, immunofluorescence, or flow cytometry to assess kinase activity in cell lines, primary cells, or tissues under experimental conditions (e.g., cytokine stimulation or inhibitor treatment). Researchers employ it to explore JAK-STAT pathway dynamics, drug efficacy in targeting JAKs, or disease mechanisms. Validation includes testing phospho-specificity via kinase inhibitors or phosphorylation-deficient mutants. Understanding JAK2/3 phosphorylation provides insights into therapeutic strategies for JAK-related disorders, including the development of selective kinase inhibitors.
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