| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase |
| Entrez GeneID | 4296; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MLK3 around the phosphorylation site of S674. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于MLK3 (Phospho-Ser674)抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
1. **"Phosphorylation of MLK3 at Ser674 mediates oxidative stress-induced activation of JNK signaling"**
- 作者：Zhang Y, et al.
- 摘要：研究通过Phospho-Ser674抗体发现，MLK3在氧化应激下通过Ser674磷酸化激活JNK通路，调控细胞凋亡和炎症反应。
2. **"A novel role of MLK3 phosphorylation in neuronal differentiation revealed by site-specific antibodies"**
- 作者：Chen L, et al.
- 摘要：利用Phospho-Ser674抗体，作者证明MLK3的磷酸化状态影响神经元分化，Ser674位点突变会抑制MAPK信号传导。
3. **"Development and validation of a phospho-specific MLK3 antibody for studying Parkinson's disease models"**
- 作者：Kim H, et al.
- 摘要：研究报道了一种高特异性Phospho-Ser674抗体的开发，并在帕金森病模型中验证其用于检测MLK3活化与神经退行性变的关系。
**注意**：以上为模拟文献，实际研究中请通过PubMed或Google Scholar以关键词“MLK3 Ser674 phosphorylation antibody”检索真实文献，并参考抗体供应商(如CST、Abcam)产品页引用的论文。
The MLK3 (Phospho-Ser674) antibody is a specialized tool used to detect the phosphorylated form of Mixed Lineage Kinase 3 (MLK3) at serine residue 674. MLK3. a serine/threonine kinase belonging to the MAPK kinase kinase (MAP3K) family, plays a crucial role in regulating stress-activated signaling pathways, including the JNK and p38 MAPK cascades. These pathways are involved in cellular responses such as apoptosis, inflammation, and differentiation. Phosphorylation at Ser674 is a critical post-translational modification that modulates MLK3’s kinase activity, autophosphorylation, and interaction with downstream signaling components. This modification is often linked to MLK3 activation in response to cellular stressors or extracellular stimuli, including cytokines and oxidative stress.
The antibody is specifically designed to recognize MLK3 only when phosphorylated at Ser674. enabling researchers to study the activation status and regulatory mechanisms of MLK3 in various biological contexts. It is widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to investigate MLK3's role in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. By targeting this phosphorylation site, the antibody provides insights into MLK3-mediated signaling dynamics, aiding in the exploration of therapeutic targets for pathologies associated with dysregulated stress-response pathways. Validation of specificity through knockout controls or phosphatase treatment is recommended to ensure accurate detection.
×
