Rabbit Polyclonal NACA(Phospho-Ser43) Antibody

The NACA (Nascent Polypeptide-Associated Complex Alpha subunit) protein is a conserved eukaryotic chaperone that binds directly to nascent polypeptide chains emerging from ribosomes, preventing their premature aggregation or misfolding during synthesis. Phosphorylation at serine 43 (Ser43) is a key post-translational modification regulating NACA's functional interactions. Studies suggest this modification may influence its role in co-translational protein quality control, stress response pathways, or its ability to partner with beta-subunits and other chaperones. The NACA (Phospho-Ser43) antibody is a monoclonal or polyclonal tool specifically designed to detect Ser43-phosphorylated NACA isoforms. It is typically validated in applications like Western blotting, immunofluorescence, or immunoprecipitation to study phosphorylation dynamics under cellular stress (e.g., heat shock, oxidative conditions) or disease states linked to proteostasis dysregulation, such as neurodegeneration or cancer. Researchers use this antibody to explore how NACA phosphorylation modulates ribosome-associated processes, substrate specificity, or its crosstalk with ubiquitin-proteasome or autophagy systems. Proper controls, including non-phosphorylated peptide blocking and knockout cell lines, are essential to confirm specificity due to potential cross-reactivity with similar phospho-epitopes.