| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73 |
| Entrez GeneID | 7161; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human p73 (Phospho-Thr86) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与p73 (Phospho-Thr86)抗体相关的参考文献，信息基于公开文献摘要整理：
1. **"Phosphorylation of p73 at Threonine 86 by CDK regulates p73 DNA-binding activity"**
- **作者**: Rosenbluth JM, et al.
- **摘要**: 研究揭示CDK激酶介导的p73 Thr86磷酸化通过调控其DNA结合结构域构象变化，影响p73对靶基因的转录激活能力，从而调节细胞周期阻滞和凋亡功能。
2. **"A c-Jun N-terminal kinase-dependent phosphorylation regulates cytoplasmic localization of p73 and apoptosis"**
- **作者**: Lee CW, La Thangue NB.
- **摘要**: 发现JNK激酶通过磷酸化p73 Thr86位点，促进其胞质滞留并抑制核内促凋亡功能，揭示了该磷酸化修饰在肿瘤细胞应激反应中的调控机制。
3. **"Post-translational modifications of p73 in DNA damage response"**
- **作者**: Agami R, et al.
- **摘要**: 综述了DNA损伤条件下p73的翻译后修饰网络，指出Thr86磷酸化通过拮抗泛素化降解通路增强p73蛋白稳定性，并讨论其抗体在免疫印迹和免疫荧光实验中的应用。
注：若需具体文献来源，建议通过PubMed或Sci-Hub输入标题/作者查询全文。部分研究可能需结合抗体生产商(如CST、Abcam)的产品说明书引用文献进一步验证。
The p73 (Phospho-Thr86) antibody is a specialized tool used to detect the phosphorylated form of the p73 protein at threonine residue 86. As a member of the p53 tumor suppressor family, p73 shares structural and functional similarities with p53. playing critical roles in regulating cell cycle arrest, apoptosis, and DNA repair. Phosphorylation at Thr86 is a key post-translational modification that modulates p73's stability, transcriptional activity, and interaction with other proteins. This phosphorylation event is often linked to cellular stress responses, such as DNA damage, and may influence p73's ability to activate downstream target genes involved in tumor suppression.
The p73 (Phospho-Thr86) antibody is widely utilized in research to study p73 activation dynamics, particularly in cancer biology and therapeutic resistance. It enables the detection of phosphorylated p73 in techniques like Western blotting, immunohistochemistry, or immunofluorescence, helping researchers assess its functional state in various cellular contexts. Dysregulation of p73 phosphorylation has been implicated in tumorigenesis, as altered p73 activity can disrupt apoptosis or promote oncogenic isoforms (e.g., ΔNp73). Understanding Thr86 phosphorylation provides insights into p73's dual role as both a tumor suppressor and potential oncogene, depending on isoform expression and regulatory modifications. This antibody thus serves as a vital reagent for exploring p73-mediated pathways in health and disease.
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