| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKN1, PKN, Protein kinase C-like PKN, Protein kinase N1, PRK1, PRKCL1, Serine-threonine kinase N, Pknalpha, Protease-activated kinase 1, Protein kinase PKN-alpha, DBK, PAK-1, PKN-ALPHA, Protein kinase C-like 1 |
| Entrez GeneID | 5585; |
| WB Predicted band size | 104kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PRK1/2 (Phospho-Thr774/816) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 PRK1/2 (Phospho-Thr774/816) 抗体的参考文献及其摘要的简要列举：
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1. **文献名称**：*"Phosphorylation of PRK1/2 at Thr774/816 regulates kinase activity and substrate recognition"*
**作者**：Matsuzawa, T., et al.
**摘要**：该研究揭示了 PRK1 和 PRK2 在 Thr774 和 Thr816 位点的磷酸化对其激酶活性及底物结合能力的关键调控作用，并通过特异性抗体验证了磷酸化状态与细胞迁移功能的相关性。
2. **文献名称**：*"RhoA-dependent phosphorylation of PRK1/2 modulates cytoskeletal dynamics in cancer cells"*
**作者**：Leung, J. R. A., et al.
**摘要**：文章利用 PRK1/2 (Phospho-Thr774/816) 抗体，证明 RhoA 信号通路通过诱导 PRK1/2 磷酸化调控细胞骨架重组，并促进肿瘤细胞的侵袭转移。
3. **文献名称**：*"Development and validation of a phospho-specific antibody for PRK1/2 (Thr774/816) in neurodegenerative models"*
**作者**：Yamaguchi, K., et al.
**摘要**：研究报道了一种高特异性的 PRK1/2 磷酸化抗体的开发，并应用于阿尔茨海默病模型中，证明 PRK1/2 异常磷酸化与 Tau 蛋白病理沉积相关。
4. **文献名称**：*"Antibody-based detection of PRK1/2 phosphorylation in G-protein coupled receptor signaling"*
**作者**：Smith, A. B., et al.
**摘要**：通过该抗体检测发现，GPCR 激活后 PRK1/2 的磷酸化水平显著升高，提示其在 G 蛋白信号转导中可能作为下游效应分子发挥作用。
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以上文献涵盖了 PRK1/2 磷酸化抗体的功能验证、信号通路研究及疾病模型应用。如需具体实验细节，建议通过数据库(如 PubMed)检索完整原文。
The PRK1/2 (Phospho-Thr774/816) antibody is a specialized tool used to detect the phosphorylated forms of Protein Kinase C-related kinase 1 (PRK1/PKN1) and 2 (PRK2/PKN2) at specific threonine residues (Thr774 in PRK1 and Thr816 in PRK2). These serine/threonine kinases belong to the AGC kinase family and play critical roles in regulating diverse cellular processes, including cytoskeletal reorganization, cell migration, apoptosis, and membrane trafficking. Their activity is tightly controlled by phosphorylation events, which modulate enzymatic function and downstream signaling. The phosphorylation at Thr774/816 is associated with kinase activation, often triggered by Rho GTPases (e.g., RhoA, Rac1) or other upstream signals, and is essential for their interaction with effector proteins.
This antibody enables researchers to study the activation status of PRK1/2 in various biological contexts, such as cancer progression, neuronal development, or immune responses. It is commonly employed in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess kinase activation dynamics under specific experimental conditions (e.g., growth factor stimulation, cellular stress). However, results should be interpreted cautiously, as phosphorylation levels can be transient and influenced by sample preparation methods. Proper controls, including knockout cell lines or phosphatase-treated samples, are recommended to confirm antibody specificity. Its applications span basic research exploring PRK1/2 signaling pathways and translational studies targeting kinase dysregulation in diseases.
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