Rabbit Polyclonal XRCC4(Phospho-Ser260) Antibody

中文名： XRCC4 (Phospho-Ser260)抗体
别名： XRCC4, DNA repair protein XRCC4

货号: IPDX41123

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	XRCC4, DNA repair protein XRCC4
Entrez GeneID	7518;
WB Predicted band size	40kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthesized peptide derived from human XRCC4 (Phospho-Ser260)
Formulation	Purified antibody in PBS with 0.05% sodium azide.

+ +

Western Blot analysis of lysates of 1. A549 cell and 2. A549 cell treated with 100ng/mL LPS for 30min, using primary antibody at 1:1000 dilution.

参考文献

以下是关于XRCC4 (Phospho-Ser260)抗体的参考文献示例(注：文献为虚构示例，实际引用需核实)：

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1. **文献名称**：*Phosphorylation of XRCC4 at Ser260 regulates DNA repair complex assembly*

**作者**：Johnson M et al.

**摘要**：本研究通过开发特异性抗XRCC4 (Phospho-Ser260)抗体，揭示了DNA-PK依赖的磷酸化修饰在DNA双链断裂修复中促进XRCC4与XLF相互作用的关键作用，Western blot和免疫荧光证实了磷酸化动态变化。

2. **文献名称**：*DNA damage-induced phosphorylation of XRCC4 enhances non-homologous end joining*

**作者**：Wang Y et al.

**摘要**：利用Phospho-Ser260特异性抗体，作者发现电离辐射诱导的XRCC4磷酸化通过增强其与DNA ligase IV的结合，提升NHEJ修复效率，突变体实验证明该位点对细胞存活至关重要。

3. **文献名称**：*A kinase-dead DNA-PKcs blocks XRCC4 phosphorylation and compromises repair*

**作者**：Thompson R et al.

**摘要**：研究通过Phospho-Ser260抗体检测发现，DNA-PK激酶活性缺失导致XRCC4磷酸化水平下降，阻碍修复复合体形成，证实DNA-PK直接调控Ser260位点修饰。

4. **文献名称**：*Cell cycle-specific phosphorylation of XRCC4 modulates replication stress response*

**作者**：Singh A et al.

**摘要**：通过时间分辨抗体分析，研究显示XRCC4在Ser260的磷酸化在S期显著上调，且与ATR信号通路协同应对复制叉崩溃，提示其双重修复调控机制。

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**注意**：以上文献为示例，实际研究中请通过数据库(如PubMed、Google Scholar)以关键词“XRCC4 Ser260 phosphorylation antibody”检索真实文献，并参考抗体供应商(如CST、Abcam)提供的引用文献列表。

背景信息

The XRCC4 (Phospho-Ser260) antibody is a specialized tool used to study the phosphorylation state of XRCC4. a critical protein in DNA repair processes. XRCC4 (X-ray repair cross-complementing protein 4) plays a central role in non-homologous end joining (NHEJ), the primary pathway for repairing DNA double-strand breaks (DSBs) in mammalian cells. It forms a stable complex with DNA ligase IV, facilitating ligation of broken DNA ends, and interacts with other repair factors like XLF and PAXX to stabilize the repair machinery.

Phosphorylation at Serine 260 (Ser260) is a post-translational modification implicated in regulating XRCC4’s function. Studies suggest this modification may influence protein-protein interactions, subcellular localization, or repair efficiency in response to DNA damage. Phosphorylation events in NHEJ components are often triggered by DNA damage sensors like ATM/ATR kinases, highlighting their role in fine-tuning repair dynamics.

The XRCC4 (Phospho-Ser260) antibody enables detection of this specific phosphorylation event, aiding research on DNA damage response mechanisms, cancer biology (where NHEJ dysregulation is common), and therapeutic interventions targeting genome stability. Its applications include Western blotting, immunofluorescence, and studying phosphorylation dynamics under genotoxic stress. Validation typically involves using cell models with induced DNA damage or phosphatase treatment to confirm phosphorylation-dependent signals.