Rabbit Polyclonal USP28(Phospho-Ser714) Antibody

The USP28 (Phospho-Ser714) antibody is designed to detect ubiquitin-specific protease 28 (USP28) when phosphorylated at serine residue 714. USP28. a member of the ubiquitin-specific protease family, functions as a deubiquitinating enzyme involved in regulating protein stability and turnover. It plays critical roles in DNA damage response, cell cycle control, and oncogenic signaling by stabilizing key substrates like c-Myc, p53. and Chk1. Phosphorylation at Ser714 is a post-translational modification linked to USP28’s activity modulation or interaction with regulatory partners. This modification may influence its cellular localization, enzymatic function, or substrate specificity in response to stress signals or kinase activation. The antibody is commonly used in research to investigate USP28’s role in pathways such as genomic stability, apoptosis, and cancer progression. Its specificity for the phosphorylated form allows detection of activated or modified USP28 in experimental models, aiding studies on phosphorylation-dependent mechanisms in disease contexts. Validation typically includes Western blotting and immunofluorescence, with controls like phosphatase-treated samples confirming phospho-specificity. Understanding USP28 phosphorylation dynamics provides insights into therapeutic targeting of deubiquitinases in cancers or disorders linked to dysregulated protein degradation.