| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SREBF2, SREBP2, SREBP-2, BHLHd2 |
| Entrez GeneID | 6721; |
| WB Predicted band size | 124kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SREBP-2 (Phospho-Ser455) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是假设的关于SREBP-2 (Phospho-Ser455)抗体的参考文献示例(注：实际文献可能需要通过数据库验证):
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1. **"Phosphorylation of SREBP-2 at Ser455 modulates its proteolytic activation and cholesterol biosynthesis"**
*Author: Zhang et al. (2017)*
摘要：研究SREBP-2在Ser455位点的磷酸化对其蛋白酶解活化的影响，通过Phospho-Ser455抗体验证高胆固醇条件下该位点的修饰，揭示其调控胆固醇合成的机制。
2. **"A phospho-specific antibody reveals insulin-induced SREBP-2 phosphorylation at Ser455 in hepatocytes"**
*Author: Tanaka et al. (2019)*
摘要：开发并验证一种针对Ser455磷酸化位点的抗体，证明胰岛素信号通过AKT通路促进SREBP-2磷酸化，并影响其核转位能力。
3. **"Validation of SREBP-2 (Phospho-Ser455) antibody for detecting cellular stress responses"**
*Author: Roberts & Clarke (2020)*
摘要：评估该抗体在氧化应激模型中的特异性，证明内质网应激可诱导Ser455磷酸化，且与脂代谢紊乱相关。
4. **"Site-specific phosphorylation of SREBP-2 regulates its interaction with SCAP and lipid homeostasis"**
*Author: Kim et al. (2018)*
摘要：利用Phospho-Ser455抗体证明该位点磷酸化阻碍SREBP-2/SCAP复合物形成，从而抑制胆固醇摄取基因的表达。
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**注意**：以上为模拟文献，实际引用时需通过PubMed、Google Scholar等平台检索真实文献(关键词：SREBP-2. Ser455 phosphorylation, antibody validation)。若需具体文献，建议补充抗体生产商提供的引用文献(如Cell Signaling Technology等公司的产品手册)。
**Background of SREBP-2 (Phospho-Ser455) Antibody**
Sterol regulatory element-binding protein 2 (SREBP-2) is a key transcription factor regulating cholesterol biosynthesis and uptake. It primarily controls genes involved in cholesterol homeostasis, such as HMG-CoA reductase and LDL receptor. SREBP-2 is synthesized as an inactive precursor bound to the endoplasmic reticulum (ER) membrane. Upon cellular cholesterol depletion, it undergoes proteolytic cleavage, releasing its N-terminal transcriptionally active domain, which translocates to the nucleus to activate target genes.
Phosphorylation at Ser455 (S455) is a critical post-translational modification modulating SREBP-2 activity. This phosphorylation event, mediated by kinases such as AMPK or Akt, influences SREBP-2 stability, nuclear localization, or transcriptional efficiency. For example, phosphorylation at S455 may promote its proteasomal degradation or inhibit processing, thereby suppressing cholesterol synthesis under energy-sufficient conditions.
The SREBP-2 (Phospho-Ser455) antibody is a specialized tool to detect this phosphorylation state, enabling researchers to study regulatory mechanisms of cholesterol metabolism in contexts like atherosclerosis, metabolic syndrome, or cancer. It is widely used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess SREBP-2 activation dynamics in response to sterol levels, insulin signaling, or pharmacological interventions (e.g., statins). Understanding SREBP-2 phosphorylation provides insights into metabolic disorders and potential therapeutic strategies targeting lipid regulation.
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