| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERM; ETV5 |
| Entrez GeneID | 2119 |
| clone | 3H3 |
| WB Predicted band size | 58kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ETV5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CD19 (Phospho-Tyr500)抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*"CD19 Phosphorylation at Tyr500 Regulates B Cell Receptor Signaling in Autoimmunity"*
**作者**：Smith et al.
**摘要**：研究通过Phospho-Tyr500抗体发现，CD19 Tyr500位点的磷酸化在B细胞受体(BCR)激活后增强，并促进下游PI3K/Akt信号通路，与小鼠自身免疫模型中B细胞过度活化相关。
2. **文献名称**：*"Dynamic Tyrosine Phosphorylation of CD19 Modulates B Cell Development"*
**作者**：Lee et al.
**摘要**：利用Phospho-Tyr500抗体进行流式细胞术分析，揭示CD19 Tyr500在骨髓B细胞成熟阶段的时序性磷酸化，影响BCR信号阈值和细胞存活。
3. **文献名称**：*"Targeting CD19 Phosphorylation in B Cell Malignancies"*
**作者**：Garcia-Rodriguez et al.
**摘要**：通过Western blot和Phospho-Tyr500抗体证实，CD19 Tyr500在弥漫大B细胞淋巴瘤(DLBCL)中异常磷酸化，抑制该位点可增强化疗药物敏感性。
(注：以上文献为模拟示例，实际引用需查询真实数据库如PubMed。)
CD19. a transmembrane glycoprotein expressed on B cells, plays a critical role in regulating B cell receptor (BCR) signaling and immune responses. It functions as a coreceptor with CD21 and CD81. modulating signal transduction pathways that influence B cell activation, proliferation, and differentiation. Phosphorylation of specific tyrosine residues within CD19’s cytoplasmic domain is essential for its activity. The phosphorylation at Tyr500 (Y500) is a key regulatory event that facilitates interactions with signaling molecules like PI3K and Vav, promoting downstream pathways such as Akt/mTOR and cytoskeletal reorganization. This post-translational modification is tightly linked to BCR engagement and co-stimulatory signals.
CD19 (Phospho-Tyr500) antibodies are valuable tools for studying B cell signaling dynamics. These antibodies specifically detect CD19 when phosphorylated at Y500. enabling researchers to investigate activation states, signaling aberrations, or therapeutic interventions in diseases like B cell malignancies (e.g., leukemia, lymphoma) and autoimmune disorders. Applications include Western blotting, immunofluorescence, and flow cytometry to assess phosphorylation levels in cell lines, primary B cells, or tissue samples.
Research using these antibodies has shed light on dysregulated BCR signaling in pathologies and the mechanisms of CD19-targeted therapies, such as CAR-T cells. Understanding Y500 phosphorylation also aids in exploring resistance mechanisms to targeted treatments. Proper validation (e.g., knockout controls, peptide competition) ensures specificity, as cross-reactivity with other phospho-tyrosine residues can occur. Overall, CD19 (Phospho-Tyr500) antibodies are pivotal for dissecting B cell biology and developing precision immunotherapies.
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