Rabbit Polyclonal LAT(Phospho-Tyr255) Antibody

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     Western blot analysis LAT (Phospho-Tyr255) using H2O2 treated 293 whole cell lysates    

The LAT (Phospho-Tyr255) antibody is a specialized tool used to study the phosphorylation status of the Linker for Activation of T-cells (LAT) protein at tyrosine residue 255. LAT is a critical transmembrane adaptor protein in T-cell receptor (TCR) signaling, facilitating the assembly of multiprotein complexes that drive downstream signaling cascades. Phosphorylation of Tyr255 occurs following TCR engagement, serving as a key regulatory event that recruits signaling molecules like PLC-γ1 and Grb2 to propagate activation signals. This modification is essential for T-cell function, including cytokine production, proliferation, and immune response coordination.

The antibody specifically detects phosphorylated LAT at Tyr255. making it invaluable for investigating T-cell activation mechanisms, immune dysregulation, and diseases such as autoimmune disorders or lymphomas. Researchers employ it in techniques like Western blotting, immunofluorescence, and flow cytometry to assess LAT activation dynamics in cellular models or patient samples. Its specificity helps differentiate active from inactive LAT, providing insights into signaling pathway modulation under experimental or pathological conditions. Understanding LAT phosphorylation is also relevant for developing targeted immunotherapies, as aberrant LAT signaling is linked to immune deficiencies and malignancies.