| WB | 咨询技术 | Human,Mouse,Rat |
| IF | IP1-2 μg per100-500 μg of total protein (1 ml of cell lysate) | Human,Mouse,Rat |
| IHC | 1/50-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P2U; HP2U; P2U1; P2UR; P2Y2; P2RU1; P2Y2R |
| Entrez GeneID | 5029 |
| clone | 4F11E7 |
| WB Predicted band size | 42.3kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human P2RY2 (AA: extra mix) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于eIF-2α (Phospho-Ser52)抗体的3篇参考文献及其摘要概括：
1. **"Regulation of translation initiation by ER stress: Role of the eIF2α kinase PERK"**
- **作者**: Harding, H.P., et al.
- **摘要**: 研究内质网(ER)应激下PERK激酶介导的eIF-2α Ser52磷酸化对翻译起始的调控，揭示其通过抑制eIF2复合体形成减少全局蛋白合成，同时选择性激活ATF4等应激反应基因。
2. **"Phosphorylation of eIF2α in Alzheimer's disease brain links Aβ toxicity with translational inhibition"**
- **作者**: O'Connor, T., et al.
- **摘要**: 在阿尔茨海默病模型中，使用Phospho-Ser52抗体发现β淀粉样蛋白(Aβ)通过激活PERK通路诱导eIF-2α磷酸化，导致神经元翻译抑制和突触功能障碍，提示其与认知衰退相关。
3. **"A conserved mechanism of eIF2α kinase activation in viral infection and cellular stress"**
- **作者**: Mohr, I., et al.
- **摘要**: 分析病毒(如HSV-1)感染后宿主细胞通过PKR激酶磷酸化eIF-2α Ser52的保守机制，证明该修饰抑制病毒蛋白翻译并促进应激颗粒形成，是抗病毒防御的关键步骤。
4. **"Targeting eIF2α phosphorylation in cancer therapy: Mechanisms and implications"**
- **作者**: Raven, J.F., et al.
- **摘要**: 综述eIF-2α Ser52磷酸化在肿瘤中的双重作用，包括通过药物(如Salubrinal)调控该修饰诱导癌细胞凋亡或增强化疗敏感性，强调其作为潜在治疗靶点的价值。
以上文献涵盖了该抗体在应激响应、神经退行性疾病、病毒感染及癌症研究中的应用。
The eIF-2α (Phospho-Ser52) antibody is a critical tool for studying the regulation of protein synthesis under cellular stress. Eukaryotic initiation factor 2 alpha (eIF-2α) is a subunit of the eIF-2 complex, which delivers the initiator Met-tRNA to ribosomes during translation initiation. Phosphorylation of eIF-2α at serine 52 (Ser51 in humans, Ser52 in some species) is a central regulatory mechanism that reduces global protein synthesis while promoting selective translation of stress-responsive mRNAs, such as ATF4. This modification is catalyzed by stress-activated kinases (e.g., PERK, PKR, HRI, GCN2) in response to diverse stressors, including ER stress, viral infection, nutrient deprivation, or oxidative damage.
The eIF-2α (Phospho-Ser52) antibody specifically detects this phosphorylation event, enabling researchers to monitor activation of the integrated stress response (ISR) pathway. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate cellular stress signaling in models of neurodegenerative diseases, cancer, viral infections, and metabolic disorders. Validation of this antibody typically includes testing in knockout cells or with phosphatase-treated lysates to confirm specificity. By detecting phosphorylated eIF-2α, researchers gain insights into how cells adapt to stress and how dysregulation of this pathway contributes to disease pathogenesis.
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