Mouse Monoclonal HER2/ErbB2(Phospho-Thr686) Antibody

The HER2/ErbB2 (Human Epidermal Growth Factor Receptor 2) is a transmembrane tyrosine kinase receptor belonging to the ErbB family, which plays a critical role in regulating cell proliferation, survival, and differentiation. Overexpression or hyperactivation of HER2 is strongly associated with aggressive cancers, particularly breast and gastric cancers. Phosphorylation of HER2 at specific residues modulates its signaling activity, influencing downstream pathways like MAPK and PI3K/Akt. The Thr686 phosphorylation site, located within the intracellular domain, is implicated in receptor activation and downstream signaling events. Antibodies targeting HER2 phosphorylated at Thr686 (p-Thr686) are essential tools for studying HER2 activation dynamics in both physiological and pathological contexts. These antibodies enable researchers to detect and quantify phosphorylation-dependent HER2 activity in cell lysates or tissue samples using techniques like Western blotting, immunohistochemistry, or immunofluorescence. In cancer research, such antibodies help assess HER2 signaling status, evaluate therapeutic responses (e.g., to HER2-targeted therapies like trastuzumab), and investigate mechanisms of drug resistance. Validation of these antibodies includes verifying specificity through knockout/knockdown controls or phosphatase treatment. Understanding HER2 phosphorylation at Thr686 contributes to elucidating its role in oncogenesis and refining personalized treatment strategies for HER2-driven malignancies.