首页 / 产品 / 蛋白 / 其他蛋白

Recombinant Human PCNA protein

  • 中文名: 抗增殖细胞核抗原(PCNA)重组蛋白
  • 别    名: PCNA;Proliferating cell nuclear antigen
货号: PA1000-5782
Price: ¥询价
数量:
大包装询价

产品详情

纯度>95%SDS-PAGE.
种属Human
靶点PCNA
Uniprot NoP12004
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-261aa
氨基酸序列MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQL TLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLA LVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRD LSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMN EPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYY LAPKIEDEEGS
预测分子量30 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PCNA重组蛋白的3篇参考文献及其摘要概括:

1. **文献名称**: *Cloning, expression, and functional characterization of recombinant human proliferating cell nuclear antigen (PCNA)*

**作者**: Kongkiatpaiboon, S. et al.

**摘要**: 该研究报道了人源PCNA基因的克隆及在大肠杆菌中的重组表达,纯化后的蛋白通过体外DNA复制实验验证了其促进DNA聚合酶活性的功能。

2. **文献名称**: *Structural basis for the interaction of PCNA with translesion synthesis DNA polymerase η*

**作者**: Hedglin, M. et al.

**摘要**: 通过X射线晶体学解析了重组PCNA蛋白与跨损伤合成DNA聚合酶η的复合物结构,揭示了PCNA在DNA损伤修复中招募特定酶类的分子机制。

3. **文献名称**: *Optimization of recombinant PCNA expression for studying eukaryotic DNA replication in vitro*

**作者**: Dieckman, L. et al.

**摘要**: 研究优化了PCNA重组蛋白在昆虫细胞中的表达和纯化方法,获得高纯度蛋白用于体外重建真核DNA复制体系,提升了复制效率分析的灵敏度。

4. **文献名称**: *PCNA ubiquitination regulates its function in DNA damage tolerance*

**作者**: Xing, G. et al.

**摘要**: 利用重组PCNA蛋白研究其泛素化修饰对DNA损伤耐受途径的调控作用,发现泛素化通过改变PCNA与修复酶的结合模式影响细胞应对复制压力的能力。

背景信息

Proliferating Cell Nuclear Antigen (PCNA) is a highly conserved, ring-shaped protein that plays a central role in DNA replication and repair across eukaryotes and archaea. Initially identified as a nuclear antigen in proliferating cells, PCNA functions as a sliding clamp that tethers DNA polymerases and other enzymes to DNA, ensuring processivity during replication. Beyond its canonical role in DNA synthesis, PCNA coordinates critical processes such as cell cycle regulation, chromatin remodeling, and damage response by serving as a docking platform for proteins involved in mismatch repair (MMR), nucleotide excision repair (NER), and translesion synthesis (TLS).

Recombinant PCNA proteins are engineered using expression systems like *E. coli* or yeast to produce purified, functional variants for structural and mechanistic studies. These recombinant versions retain the ability to form homotrimers and interact with binding partners through conserved motifs like the PIP-box (PCNA-Interacting Protein box). Researchers utilize them to dissect replication fork dynamics, study protein interaction networks, or develop assays for drug discovery. For example, fluorescently labeled recombinant PCNA enables real-time visualization of replication in single-molecule experiments.

The development of mutant or tagged recombinant PCNA (e.g., His-tagged, SUMOylated) has advanced our understanding of post-translational modifications regulating its functions. In therapeutic contexts, PCNA-targeting peptides are explored for cancer treatment due to their role in maintaining genomic stability in rapidly dividing cells. Its conservation and multifunctionality make recombinant PCNA a cornerstone for investigating genome maintenance mechanisms and designing targeted molecular therapies.

客户数据及评论

折叠内容

大包装询价

×