Recombinant Human APLP1 protein

中文名： 淀粉样蛋白β前体样蛋白1(APLP1)重组蛋白
别名： APLP1；Amyloid beta precursor like protein 1

货号: PA1000-5880

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	APLP1
Uniprot No	P51693-1
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-580aa
氨基酸序列	MGPASPAARG LSRRPGQPPL PLLLPLLLLL LRAQPAIGSL AGGSPGAAEA PGSAQVAGLC GRLTLHRDLR TGRWEPDPQR SRRCLRDPQR VLEYCRQMYP ELQIARVEQA TQAIPMERWC GGSRSGSCAH PHHQVVPFRC LPGEFVSEAL LVPEGCRFLH QERMDQCESS TRRHQEAQEA CSSQGLILHG SGMLLPCGSD RFRGVEYVCC PPPGTPDPSG TAVGDPSTRS WPPGSRVEGA EDEEEEESFP QPVDDYFVEP PQAEEEEETV PPPSSHTLAV VGKVTPTPRP TDGVDIYFGM PGEISEHEGF LRAKMDLEER RMRQINEVMR EWAMADNQSK NLPKADRQAL NEHFQSILQT LEEQVSGERQ RLVETHATRV IALINDQRRA ALEGFLAALQ ADPPQAERVL LALRRYLRAE QKEQRHTLRH YQHVAAVDPE KAQQMRFQVH THLQVIEERV NQSLGLLDQN PHLAQELRPQ IQELLHSEHL GPSELEAPAP GGSSEDKGGL QPPDSKDDTP MTLPKGSTEQ DAASPEKEKM NPLEQYERKV NASVPRGFPF HSSEIQRDEL APAGTGVSRE
预测分子量	62 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于APLP1重组蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库检索)：

1. **《Recombinant Expression and Functional Characterization of APLP1 in Neuronal Cell Adhesion》**

- 作者：Smith J et al.

- 摘要：研究通过哺乳动物细胞系统表达APLP1重组蛋白，验证其促进神经元细胞黏附的功能，并证明其与整合素受体的相互作用。

2. **《Purification and Structural Analysis of APLP1 Extracellular Domain in E. coli》**

- 作者：Zhang L et al.

- 摘要：利用原核表达系统优化APLP1胞外结构域的重组表达和纯化，结合X射线晶体学解析其三维结构，揭示与APP蛋白的相似性。

3. **《APLP1 Knockout Mice Exhibit Altered Synaptic Plasticity and Cognitive Deficits》**

- 作者：Jones R et al.

- 摘要：通过构建APLP1基因敲除小鼠模型，发现APLP1缺失导致突触可塑性下降和认知障碍，提示其在神经退行性疾病中的潜在作用。

4. **《APLP1 Recombinant Protein Inhibits Aβ Aggregation in Alzheimer’s Disease Models》**

- 作者：Wang Y et al.

- 摘要：体外实验表明APLP1重组蛋白可抑制β-淀粉样蛋白(Aβ)的聚集，为阿尔茨海默病的治疗提供新靶点。

**提示**：建议通过PubMed、Web of Science等平台，以关键词“APLP1 recombinant protein”或“Amyloid precursor-like protein 1 expression”检索最新文献。注意优先选择近5年发表且高被引的研究。

背景信息

**Background of APLP1 Recombinant Protein**

Amyloid Precursor-Like Protein 1 (APLP1) is a transmembrane glycoprotein belonging to the amyloid precursor protein (APP) family, which includes APP and APLP2. While APP is widely studied for its role in Alzheimer’s disease due to its cleavage into amyloid-β peptides, APLP1 shares structural homology but lacks the amyloidogenic domain, preventing β-amyloid formation. APLP1 is predominantly expressed in the nervous system, particularly in neurons, and plays critical roles in synaptic maintenance, cell adhesion, and neuronal signaling.

Recombinant APLP1 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper post-translational modifications. The recombinant form often includes tags (e.g., His-tag) for purification and detection. Studies using APLP1 recombinant protein focus on elucidating its physiological functions, such as interactions with synaptic proteins (e.g., neurexins) or extracellular matrix components, which are vital for neural network stability.

APLP1 knockout mouse models exhibit synaptic deficits and behavioral abnormalities, underscoring its importance in neurodevelopment. In research, recombinant APLP1 aids in structural-functional analyses, ligand-receptor binding assays, and exploration of its non-amyloidogenic pathways. Its role in diseases remains less defined than APP’s, but emerging evidence links APLP1 dysregulation to neurological disorders and cancer epithelial-mesenchymal transition (EMT).

Overall, APLP1 recombinant protein serves as a key tool to decode its biological significance, offering insights into neural health and potential therapeutic targets.