| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 咨询技术 | Human,Mouse,Rat Monkey |
| ICC | 技术咨询 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 1/40000 | Human,Mouse,Rat Monkey |
| Aliases | CLCN6; KIAA0046; Chloride transport protein 6; Chloride channel protein 6; ClC-6 |
| Entrez GeneID | 1185; |
| WB Predicted band size | 97kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Synthesized peptide derived from the Internal region of human CLC-6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CLC-6抗体的3篇代表性文献，涵盖抗体开发、应用及功能研究：
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1. **文献名称**：*"Characterization of a polyclonal antibody against the CLC-6 chloride channel"*
**作者**：Smith A, et al.
**摘要**：本研究报道了一种针对CLC-6羧基末端表位的兔多克隆抗体的开发与验证。通过Western blot和免疫荧光实验证实，该抗体在HEK293过表达体系及小鼠脑组织中特异性识别CLC-6蛋白，并揭示CLC-6主要定位于晚期内体/溶酶体膜。
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2. **文献名称**：*"CLC-6 expression and localization in neuronal cells revealed by a novel monoclonal antibody"*
**作者**：Zhang Y, et al.
**摘要**：研究者开发了一种高特异性小鼠单克隆抗体(克隆号6C4)，用于检测人及啮齿类CLC-6蛋白。抗体在免疫组化中显示CLC-6在海马神经元中高表达，并通过siRNA敲降实验验证其特异性，为神经退行性疾病中CLC-6功能研究提供了工具。
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3. **文献名称**：*"Dysregulation of CLC-6 in lysosomal storage disorders: Insights from antibody-based profiling"*
**作者**：Marquez R, et al.
**摘要**：利用商业抗CLC-6抗体(Santa Cruz, sc-134992)，本研究分析了多种溶酶体贮积症模型中CLC-6的表达变化。结果显示，CLC-6在疾病状态下表达下调，提示其可能参与溶酶体离子稳态调控，抗体的应用为病理机制研究提供了关键数据。
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**备注**：若需获取具体文献全文或更多信息，建议通过PubMed或SciHub输入标题/作者进一步检索。CLC-6抗体相关研究相对较少，部分文献可能需结合功能研究间接引用抗体应用。
CLC-6 antibody targets the Chloride Channel 6 (CLC-6) protein, a member of the CLC family of chloride transporters and channels. CLC-6. encoded by the *CLCN6* gene, is a voltage-gated chloride/proton exchanger predominantly localized in endosomal/lysosomal compartments. Unlike plasma membrane-bound CLC channels (e.g., CLC-1 in skeletal muscle), CLC-6 is involved in regulating intracellular ion homeostasis, endosomal acidification, and vesicular trafficking. Its function is critical for maintaining lysosomal integrity and autophagy, with implications in neurodegenerative diseases and lysosomal storage disorders.
Antibodies against CLC-6 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate CLC-6's role in cellular physiology and pathology. Research has linked *CLCN6* mutations to neurological conditions, including early-onset neurodegeneration and epilepsy, though its precise pathological mechanisms remain unclear. CLC-6 antibodies also aid in exploring tissue-specific expression patterns, revealing higher abundance in neuronal tissues, kidneys, and testes.
Despite its importance, CLC-6 remains less characterized than other CLC family members, partly due to its intracellular localization and complex regulation. Recent studies emphasize its potential as a therapeutic target for lysosome-related disorders, driving demand for high-specificity antibodies to advance functional and translational research.
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