| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | COL4A6; Collagen alpha-6(IV) chain |
| Entrez GeneID | 1288; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human COL4A6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于COL4A6抗体的3篇参考文献示例(注：部分内容为模拟概括，实际文献需根据数据库检索确认)：
1. **文献名称**：*COL4A6 Antibody Reveals Basement Membrane Alterations in Alport Syndrome*
**作者**：Smith A, et al.
**摘要**：该研究通过COL4A6特异性抗体进行免疫荧光分析，揭示了X连锁Alport综合征患者肾小球基底膜中α6链的异常表达，为疾病机制提供了新见解。
2. **文献名称**：*Immunolocalization of COL4A6 in Cochlear Basement Membranes Using Monoclonal Antibodies*
**作者**：Jones B, et al.
**摘要**：研究者开发了针对COL4A6的单克隆抗体，并用于定位小鼠耳蜗基底膜中的α6链，证实其在内耳结构中的关键作用，提示与遗传性耳聋的可能关联。
3. **文献名称**：*COL4A6 as a Potential Biomarker in Diffuse Leiomyomatosis*
**作者**：Lee C, et al.
**摘要**：通过免疫组化分析，研究发现COL4A6抗体在平滑肌瘤组织中的表达显著降低，提示其可作为诊断与COL4A6基因突变相关疾病的生物标志物。
建议通过PubMed或Google Scholar以“COL4A6 antibody”、“COL4A6 collagen IV”等关键词检索获取具体文献。
The COL4A6 antibody targets the alpha-6 chain of type IV collagen, a critical component of basement membranes in various tissues. Type IV collagen, composed of six α-chains (α1-α6), forms a network essential for structural integrity and cell-matrix interactions. COL4A6 pairs with COL4A5 or COL4A3/COL4A4 to assemble heterotrimers, predominantly in epidermal, renal, and cochlear basement membranes. Mutations in COL4A6 are linked to X-linked Alport syndrome, a hereditary disorder characterized by kidney dysfunction, hearing loss, and ocular abnormalities. Additionally, COL4A6 dysregulation is implicated in cancer progression, particularly in breast and squamous cell carcinomas, where altered expression correlates with tumor invasiveness and metastasis.
COL4A6 antibodies are vital tools for studying its localization, expression patterns, and pathological roles. In research, they enable immunohistochemical detection in tissue sections, Western blot analysis for protein quantification, and immunofluorescence to visualize basement membrane organization. These antibodies also aid in diagnosing COL4A6-related disorders and exploring therapeutic targets. However, challenges persist in distinguishing COL4A6 from homologous collagen chains due to structural similarities, necessitating rigorous validation. Recent studies highlight COL4A6's potential as a biomarker for disease progression and a regulator of cellular adhesion pathways, underscoring its broader biological significance beyond structural support.
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