Recombinant Human CTNNB1 protein

中文名： 连环蛋白β-1(CTNNB1)重组蛋白
别名： CTNNB1；CTNNB；Catenin beta-1

货号: PA1000-6637

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CTNNB1
Uniprot No	P35222
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-781aa
氨基酸序列	MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKG NPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP ETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELAT RAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVR TMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDS VLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDC LQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVC SSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEG LLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRT VLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHP PSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRR TSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFV QLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEG VATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDI GAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPV DGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL
预测分子量	112 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CTNNB1重组蛋白的3篇参考文献示例(注：文献信息为示例性质，非真实存在，仅供格式参考)：

1. **《Expression and Functional Analysis of Recombinant CTNNB1 in Wnt Signaling Pathway》**

- **作者**: Smith A, et al.

- **摘要**: 本研究通过大肠杆菌系统成功表达并纯化人源CTNNB1重组蛋白，验证其与TCF4转录因子的结合能力，揭示其在Wnt信号通路中的核心调控作用。

2. **《Crystal Structure of Recombinant CTNNB1 Reveals Key Interaction Domains with APC Protein》**

- **作者**: Lee B, et al.

- **摘要**: 利用X射线晶体学解析CTNNB1重组蛋白的三维结构，发现其与结肠腺瘤性息肉病(APC)蛋白结合的特定结构域，为肿瘤发生机制提供结构生物学依据。

3. **《High-Yield Production of CTNNB1 Recombinant Protein for Drug Screening Applications》**

- **作者**: Zhang C, et al.

- **摘要**: 开发了一种高效表达CTNNB1重组蛋白的哺乳动物细胞系统，应用于高通量药物筛选，鉴定出多个靶向β-catenin的小分子抑制剂。

4. **《CTNNB1 Recombinant Protein Facilitates In Vitro Modeling of Hepatocellular Carcinoma》**

- **作者**: Johnson D, et al.

- **摘要**: 通过重组CTNNB1蛋白激活肝癌细胞系中的Wnt通路，模拟肿瘤微环境，为肝癌靶向治疗研究提供体外模型。

如需真实文献，建议通过PubMed或Google Scholar以“CTNNB1 recombinant protein”为关键词检索，并筛选近年的功能研究、结构分析或应用类论文。

背景信息

**Background of CTNNB1 Recombinant Protein**

CTNNB1 encodes β-catenin, a multifunctional protein pivotal in cell-cell adhesion and Wnt signaling. As a key effector of the canonical Wnt pathway, β-catenin regulates gene expression by interacting with T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factors, driving processes like embryogenesis, tissue homeostasis, and stem cell maintenance. Its cytoplasmic levels are tightly controlled by the destruction complex (APC, AXIN, GSK3β), which phosphorylates β-catenin for proteasomal degradation. Dysregulation of CTNNB1. through mutations or Wnt pathway activation, leads to β-catenin accumulation, promoting oncogenesis in cancers such as colorectal carcinoma and hepatocellular carcinoma.

Recombinant CTNNB1 protein is engineered for in vitro studies to dissect Wnt signaling mechanisms, protein interactions, and therapeutic targeting. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it often includes tags (e.g., His, GST) for purification and detection. Researchers utilize it to explore β-catenin’s dual roles: as a cytoskeletal component binding E-cadherin in adherens junctions, and as a nuclear co-activator of proliferation-associated genes. Mutant variants (e.g., phosphorylation-resistant forms) help model pathogenic signaling.

Its applications span structural studies, drug screening (e.g., inhibitors blocking β-catenin/TCF interactions), and regenerative medicine, given Wnt’s role in tissue repair. However, challenges include maintaining post-translational modifications critical for function. CTNNB1 recombinant proteins remain vital tools for unraveling cancer biology and developing targeted therapies against Wnt-driven diseases.