| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGPAT1 |
| Uniprot No | Q99943 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MDLWPGAWMLLLLLFLLLLFLLPTLWFCSPSAKYFFKMAFYNGWILFLAVLAIPVCAVRGRNVENMKILRLMLLHIKYLYGIRVEVRGAHHFPPSQPYVVVSNHQSSLDLLGMMEVLPGRCVPIAKRELLWAGSAGLACWLAGVIFIDRKRTGDAISVMSEVAQTLLTQDVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPIVPIVMSSYQDFYCKKERRFTSGQCQVRVLPPVPTEGLTPDDVPALADRVRHSMLTVFREISTDGRGGGDYLKKPGGGG |
| 分子量 | 58.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人磷酸酰基磷酸转移酶α(AGPAT1)的简要参考文献示例：
1. **"Functional Characterization of Human AGPAT1: Role in Phospholipid Biosynthesis"**
*作者：Agarwal et al. (2006)*
**摘要**：研究通过重组表达人AGPAT1.证实其催化溶血磷脂酸(LPA)向磷脂酸(PA)的转化能力，并揭示该酶在甘油三酯和磷脂代谢中的关键作用。
2. **"Structural Insights into AGPAT1 Substrate Specificity Using Crystallography"**
*作者：Leung et al. (2012)*
**摘要**：通过X射线晶体学解析重组AGPAT1的三维结构，阐明其酰基转移活性的底物结合区域，为设计选择性抑制剂提供理论依据。
3. **"AGPAT1 Deficiency in Mice Alters Lipid Droplet Formation and Adipose Tissue Homeostasis"**
*作者：Takeuchi & Reue (2018)*
**摘要**：AGPAT1敲除小鼠模型显示其脂肪组织甘油三酯含量显著降低，证明AGPAT1在脂质储存中的生理重要性及其与代谢综合征的潜在关联。
4. **"AGPAT1 Gene Variants and Their Association with Human Insulin Resistance"**
*作者：Zhang et al. (2020)*
**摘要**：临床研究发现AGPAT1基因多态性与胰岛素抵抗和2型糖尿病相关，提示其在脂质代谢异常引发疾病中的调控作用。
(注：以上文献信息为概括性示例，非真实引用，仅用于展示回答格式。)
**Background of Recombinant Human Acyltransferase α (AGPAT1):**
AGPAT1 (1-acylglycerol-3-phosphate O-acyltransferase 1) is a key enzyme in glycerolipid metabolism, catalyzing the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a central precursor for phospholipid and triglyceride synthesis. It belongs to the AGPAT family, which comprises isoforms with distinct tissue expression and functional roles. AGPAT1 is ubiquitously expressed, with notable activity in the liver, adipose tissue, and kidneys.
This enzyme localizes to the endoplasmic reticulum and utilizes acyl-CoA donors to acylate the *sn-2* position of LPA, influencing lipid storage, membrane structure, and signaling pathways. Dysregulation of AGPAT1 has been linked to metabolic disorders, including lipodystrophy, insulin resistance, and non-alcoholic fatty liver disease (NAFLD), highlighting its physiological significance.
Recombinant AGPAT1. produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables structural and functional studies to dissect its enzymatic mechanisms and interaction networks. Its recombinant form is crucial for high-throughput screening of modulators, aiding drug discovery for metabolic diseases. Recent research also explores its role in cancer cell survival via lipid remodeling, expanding its therapeutic relevance. Overall, AGPAT1 remains a pivotal target for understanding lipid metabolism and developing precision therapies.
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