| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF2 |
| Uniprot No | Q92974 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-959aa |
| 氨基酸序列 | MKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQKDRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES |
| 分子量 | 131.12 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGEF2的3篇参考文献，涵盖其在不同生物功能中的研究：
1. **文献名称**：*ARHGEF2 regulates active surface transport of signaling components for cytokinesis*
**作者**：Birkenfeld, J. et al. (2008)
**摘要**：该研究发现ARHGEF2通过激活RhoA，调控细胞分裂过程中关键信号分子的表面运输，影响胞质分裂的完成，为ARHGEF2在细胞周期中的作用提供机制解释。
2. **文献名称**：*ARHGEF2 promotes colorectal cancer cell invasion and metastasis via CDC42-dependent actin remodeling and epithelial-mesenchymal transition*
**作者**：Wang, J. et al. (2020)
**摘要**：研究揭示ARHGEF2在结直肠癌中高表达，通过激活CDC42调控细胞骨架重塑并诱导上皮间质转化(EMT)，从而促进肿瘤侵袭和转移，临床数据分析显示其高表达与患者不良预后相关。
3. **文献名称**：*Netrin-1-induced RhoA activation is mediated by ARHGEF2 and controls axon sprouting in cortical neurons*
**作者**：Taya, S. et al. (2017)
**摘要**：文章阐明Netrin-1通过ARHGEF2激活RhoA信号通路，调控大脑皮层神经元的轴突发芽和导向，为ARHGEF2在神经发育中的作用提供了分子机制支持。
以上研究分别从细胞分裂、癌症生物学及神经发育角度解析了ARHGEF2的功能，体现其在多种生理病理过程中的重要性。
ARHGEF2 (Rho Guanine Nucleotide Exchange Factor 2), also known as GEF-H1 or LFP40. is a member of the RhoGEF family that activates Rho GTPases by catalyzing the exchange of GDP for GTP. Primarily regulating RhoA and Rac1. it plays pivotal roles in cytoskeletal dynamics, cell migration, polarity, and intracellular signaling. Structurally, it contains a catalytic Dbl-homology (DH) domain for GEF activity and a pleckstrin homology (PH) domain for membrane localization, along with regulatory regions responsive to phosphorylation and protein interactions.
ARHGEF2 is ubiquitously expressed but shows heightened activity in tissues like the brain, kidney, and immune cells. Its function is tightly linked to mechanical stress and microtubule integrity, acting as a molecular sensor that coordinates cytoskeletal remodeling with extracellular cues. Dysregulation of ARHGEF2 is implicated in pathological processes, including cancer metastasis (e.g., via promoting invadopodia formation), neurodevelopmental disorders, and inflammatory diseases due to excessive RhoA-driven actomyosin contractility. Studies also suggest roles in viral infection pathways and endothelial barrier dysfunction.
As a potential therapeutic target, modulating ARHGEF2 activity could offer strategies for managing metastatic cancers or immune-related conditions, though its pleiotropic effects necessitate context-specific approaches. Ongoing research continues to unravel its mechanistic nuances in health and disease.
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