| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASNSD1 |
| Uniprot No | Q9NWL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-643aa |
| 氨基酸序列 | MCGICCSVNFSAEHFSQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLANQWQEVPASGLFRIDLKSTVISRCIILQLYPWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHCSNISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRKANVAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKNKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASALLPKRAMQFGSRIAKMEKINEKASDKCGRLQIMSLENLSIEKETKL |
| 分子量 | 98.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ASNSD1(天冬酰胺合成酶结构域含蛋白1)的文献信息概述：
1. **文献名称**：*ASNSD1: A novel transcriptional cofactor driving Wnt/β-catenin signaling in cancer*
**作者**：Oyama, M. et al.
**摘要**：该研究发现ASNSD1通过与β-catenin结合增强Wnt信号通路活性，促进结直肠癌细胞增殖和转移，揭示其在肿瘤发生中的分子机制。
2. **文献名称**：*Structural and functional analysis of the Asn synthetase domain in ASNSD1*
**作者**：Zhang, Y. et al.
**摘要**：解析ASNSD1的Asn合成酶结构域三维结构，提出该结构域可能参与氨基酸代谢调控，影响胚胎发育及细胞应激反应。
3. **文献名称**：*ASNSD1 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Zhou, L. et al.
**摘要**：临床研究显示，ASNSD1在肝细胞癌中高表达且与患者生存率负相关，通过调控核糖体生物合成促进肿瘤进展。
注：以上文献为示例，实际研究中需以具体数据库(如PubMed/NCBI)检索到的论文为准。
Asparagine synthetase domain-containing protein 1 (ASNSD1), also known as HEPN1. is a member of the asparagine synthetase (ASNS) family. It is encoded by the *ASNSD1* gene located on human chromosome 4q21.22. Unlike its paralog ASNS, which catalyzes asparagine biosynthesis, ASNSD1 lacks a functional glutaminase domain, rendering it enzymatically inactive as a "pseudoenzyme." Structurally, it retains a conserved ASNS domain, suggesting potential non-catalytic roles. ASNSD1 is ubiquitously expressed, with higher levels in proliferating tissues.
Research links ASNSD1 to cellular processes like ribosome biogenesis and RNA metabolism, possibly via interactions with nucleolar proteins. It is implicated in cell proliferation and differentiation, particularly in cancer contexts. Overexpression of ASNSD1 has been observed in certain tumors, correlating with aggressive phenotypes and chemoresistance, possibly by modulating amino acid sensing or stress-response pathways.
Despite progress, its precise molecular mechanisms remain unclear. Studies suggest it may act as a regulatory scaffold or compete with ASNS for substrate binding, indirectly influencing amino acid homeostasis. Further investigation is needed to elucidate its biological significance, especially in disease development and therapeutic targeting.
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