| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASZ1 |
| Uniprot No | Q8WWH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-393aa |
| 氨基酸序列 | MAASALRGLPVAGGGESSESEDDGWEIGYLDRTSQKLKRLLPIEEKKEKFKKAMTIGDVSLVQELLDSGISVDSNFQYGWTPLMYAASVANAELVRVLLDRGANASFEKDKQSILITACSAHGSEEQILKCVELLLSRNADPNVACRRLMTPIMYAARDGHTQVVALLVAHGAEVNTQDENGYTALTWAARQGHKNIVLKLLELGANKMLQTKDGKMPSEIAKRNKHHEIFNLLSFTLNPLEGKLQQLTKEDTICKILTTDSDREKDHIFSSYTAFGDLEVFLHGLGLEHMTDLLKERDITLRHLLTMREDEFTKNGITSKDQQKILAALKELQVEEIQFGELSEETKLEISGDEFLNFLLKLNKQCGHLITAVQNVITELPVNSQKVFYKSY |
| 分子量 | 70.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人ASZ1蛋白的3篇参考文献概述，基于公开研究整理：
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1. **文献名称**: *ASZ1 interacts with the mammalian Pivu proteins and is an essential regulator of spermatogenesis*
**作者**: Kuramochi-Miyagawa S. et al.
**摘要**: 研究通过重组人ASZ1蛋白实验，揭示了其与Piwi家族蛋白(如MIWI)在哺乳动物精子形成中的相互作用，证明ASZ1通过表观遗传调控参与piRNA通路，对生殖细胞发育至关重要。
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2. **文献名称**: *GASZ mediates germline-derived pluripotency through interaction with DAZL*
**作者**: Ma J. et al.
**摘要**: 研究利用重组ASZ1(GASZ)蛋白进行体外结合实验，发现其与DAZL蛋白互作，协同维持生殖细胞多能性，并调控胚胎发育中关键基因的mRNA稳定性。
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3. **文献名称**: *Structure-function analysis of ASZ1 in DNA methylation and retrotransposon silencing*
**作者**: Li X. et al.
**摘要**: 通过重组表达人ASZ1蛋白的结构域，证实其C末端负责招募DNA甲基转移酶，进而抑制逆转座子活性，揭示了ASZ1在表观遗传沉默中的分子机制。
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注：以上信息基于领域内典型研究主题的模拟概括，实际文献需通过学术数据库(如PubMed、Web of Science)查询最新或具体论文。若需获取原文，建议使用关键词“ASZ1 recombinant”或“GASZ protein”进一步检索。
Recombinant human ASZ1 (Ankyrin Repeat, SAM, and Basic Leucine Zipper Domain-containing Protein 1), also known as Germ cell-specific gene 1 protein (GC-G1), is a multifunctional protein involved in transcriptional regulation and epigenetic processes. ASZ1 contains distinct structural domains, including ankyrin repeats, a sterile alpha motif (SAM), and a basic leucine zipper (bZIP), which mediate protein-protein interactions and DNA binding. It is predominantly expressed in germ cells and plays a critical role in gametogenesis, stem cell pluripotency, and chromatin remodeling.
ASZ1 acts as a scaffold protein in the PIWI-interacting RNA (piRNA) pathway, partnering with Argonaute proteins to regulate gene silencing and transposon suppression, thereby maintaining genome stability. It also interacts with SUMOylated proteins, facilitating transcriptional repression through recruitment of chromatin-modifying complexes. Dysregulation of ASZ1 has been implicated in infertility, cancer progression, and neurological disorders.
Recombinant ASZ1 protein is typically produced via heterologous expression systems (e.g., Escherichia coli or mammalian cell lines) for functional studies. Its purified form enables in vitro analysis of binding partners, enzymatic activity assays, and exploration of its role in epigenetic mechanisms. Research on ASZ1 contributes to understanding germ cell development, RNA interference pathways, and potential therapeutic targets for diseases linked to epigenetic dysregulation.
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