| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASTE1 |
| Uniprot No | Q2TB18 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679aa |
| 氨基酸序列 | MGIRGLMSFV EDHSNEFFTD LKLRDTKIVI DGYALFHRLC FSSNLDLRYG GDYDSFADVV QKFFESLFAC NICPYVVLDG GCDISDKKLT TLKDRAREKI QMAHSLSVGG SGYVCPLLIR EVFIQVLIKL RVCFVQCFSE ADRDIMTLAN HWNCPVLSSD SDFCIFDLKT GFCPLNSFQW RNMNTIKGTQ NYIPAKCFSL DAFCHHFSNM NKALLPLFAV LCGNDHVNLP IMETFLSKAR LPLGATSSKG RRHHRILGLL NWLSHFANPT EALDNVLKYL PKKDRENVKE LLCCSMEEYQ QSQVKLQDFF QCGTYVCPDA LNLGLPEWVL VALAKGQLSP FISDALVLRR TILPTQVENM QQPNAHRISQ PIRQIIYGLL LNASPHLDKT SWNALPPQPL AFSEVERINK NIRTSIIDAV ELAKDHSDLS RLTELSLRRR QMLLLETLKV KQTILEPIPT SLKLPIAVSC YWLQHTETKA KLHHLQSLLL TMLVGPLIAI INSPGKEELQ EDGAKMLYAE FQRVKAQTRL GTRLDLDTAH IFCQWQSCLQ MGMYLNQLLS TPLPEPDLTR LYSGSLVHGL CQQLLASTSV ESLLSICPEA KQLYEYLFNA TRSYAPAEIF LPKGRSNSKK KRQKKQNTSC SKNRGRTTAH TKCWYEGNNR FGLLMVENLE EHSEASNIE |
| 分子量 | 103.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人蛋白ASTE1的部分研究文献概要，基于现有知识整理(注：文献信息为虚拟示例，实际需查询最新数据库)：
1. **文献名称**: *ASTE1 regulates recombination-associated DNA damage repair in human cells*
**作者**: Zhang L, et al.
**摘要**: 研究发现ASTE1作为DNA修复酶，通过调控同源重组修复途径维持基因组稳定性，其缺失导致染色体异常和细胞周期停滞。
2. **文献名称**: *Structural and functional analysis of the human ASTE1 protein*
**作者**: Kimura T, et al.
**摘要**: 通过体外重组表达ASTE1蛋白并解析其晶体结构，揭示了其与核酸相互作用的活性位点，为探索其在DNA代谢中的机制提供依据。
3. **文献名称**: *ASTE1 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**: Wang Y, et al.
**摘要**: 临床数据分析显示，ASTE1在肝癌组织中高表达，可能通过促进异常DNA修复加速肿瘤进展，提示其作为潜在治疗靶点的可能性。
4. **文献名称**: *Interaction network of ASTE1 with replication stress response proteins*
**作者**: Gómez-Herreros F, et al.
**摘要**: 蛋白质互作研究表明，ASTE1与ATR、Rad51等复制应激应答蛋白存在功能协同，参与解决复制叉停滞并维持细胞存活。
**建议**：通过PubMed/Web of Science检索最新文献，使用关键词“ASTE1 human”、“recombinant ASTE1”、“ASTE1 function”获取更具体研究。
ASTE1 (Asteroid homolog 1), also known as C1orf112. is a conserved eukaryotic protein encoded by the *ASTE1* gene in humans. It was initially identified through homology searches as a member of the "asteroid" protein family, named for its sequence similarity to proteins in yeast and other species. ASTE1 is characterized by an OB-fold domain, a structural motif commonly associated with nucleic acid binding and protein interactions.
Functionally, ASTE1 plays a role in DNA repair and genome maintenance, particularly in the context of homologous recombination (HR). Studies link it to the resolution of DNA replication stress and the stabilization of stalled replication forks. It interacts with key DNA repair proteins like RAD51 and the MRE11 nuclease, suggesting involvement in processing DNA double-strand breaks or recombination intermediates. Emerging evidence also implicates ASTE1 in meiosis, where it may contribute to crossover formation during gametogenesis.
Dysregulation of ASTE1 has been observed in cancers, with elevated expression correlated to genomic instability and poor prognosis in certain malignancies. Conversely, its depletion sensitizes cells to DNA-damaging agents, highlighting its potential as a therapeutic target. Despite progress, the precise molecular mechanisms and full spectrum of ASTE1’s biological roles remain under investigation, warranting further exploration of its contributions to genome integrity and disease.
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