| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5G3 |
| Uniprot No | P48201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MFACAKLACTPSLIRAGSRVAYRPISASVLSRPEASRTGEGSTVFNGAQNGVSQLIQREFQTSAISRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM |
| 分子量 | 41.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ATP合成酶脂质结合蛋白(ATP5G3)的3篇参考文献的简要总结，涵盖结构、功能及疾病相关性研究：
1. **"Structural Insights into Human ATP Synthase Subunit c (ATP5G3) and Its Role in Proton Translocation"**
- 作者：A. Smith et al.
- 摘要：通过冷冻电镜技术解析了ATP5G3的跨膜螺旋结构，揭示了其参与线粒体ATP合酶质子通道形成的分子机制，并验证了重组表达蛋白在脂质体模型中的质子泵功能。
2. **"ATP5G3 Overexpression Promotes Mitochondrial Metabolism and Cancer Cell Proliferation"**
- 作者：B. Chen & R. Wang
- 摘要：研究证明，重组ATP5G3在肿瘤细胞中的过表达显著增强线粒体氧化磷酸化活性和ATP生成，促进癌细胞增殖，提示其作为癌症治疗潜在靶点的可能性。
3. **"Lipid Binding Properties of Recombinant ATP5G3 in Membrane Remodeling"**
- 作者：M. Tanaka et al.
- 摘要：利用重组人ATP5G3蛋白结合脂质体的实验，证实该亚基与心磷脂等线粒体膜脂质的特异性相互作用，并对其维持ATP合酶复合体稳定性至关重要。
以上内容基于领域内典型研究方向模拟，实际文献需通过PubMed或SciFinder等平台检索确认。
ATP synthase, H+ transporting, mitochondrial F0 complex subunit C3 (ATP5G3), also known as subunit c or proteolipid c, is a key component of the mitochondrial ATP synthase complex (Complex V). This enzyme catalyzes ATP production during oxidative phosphorylation by utilizing the proton gradient across the inner mitochondrial membrane. ATP5G3 specifically contributes to the formation of the F0 proton channel, a membrane-embedded region critical for proton translocation. As one of three isoforms (ATP5G1-3) in mammals, ATP5G3 is encoded by nuclear DNA and imported into mitochondria.
The subunit c family is evolutionarily conserved and plays a structural role in stabilizing the rotary mechanism of ATP synthase. Dysregulation or mutations in ATP5G3 have been implicated in mitochondrial disorders, metabolic syndromes, and cancers due to disrupted cellular energy metabolism. For instance, reduced ATP5G3 expression correlates with poor prognosis in certain tumors, potentially linked to the Warburg effect. Recombinant ATP5G3 proteins are often generated to study its biophysical interactions, particularly with phospholipids essential for proton channel assembly and function. Recent research also explores its role in non-canonical processes, including mitochondrial permeability transition pore regulation. Structural studies using recombinant ATP5G3 provide insights into its lipid-binding specificity and pathogenic variants, advancing therapeutic strategies targeting mitochondrial bioenergetics.
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