首页 / 产品 / 蛋白 / 信号通路蛋白

Recombinant Human CCNA1 protein

  • 中文名: 细胞周期素A1(CCNA1)重组蛋白
  • 别    名: CCNA1;Cyclin-A1
货号: PA1000-7084
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点CCNA1
Uniprot No P78396
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 1-464aa
氨基酸序列METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVFQQQPVESEAMHCSNPKSGVVLATVARGPDACQILTRAPLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDELEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEYAEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSVLRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQYLRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVPCLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLL
预测分子量 54.2kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CCNA1重组蛋白的参考文献示例(注:部分内容为示例性概括,非真实文献):

---

1. **文献名称**:*Expression and Purification of Recombinant Cyclin A1 (CCNA1) in E. coli*

**作者**:Smith, J., et al.

**摘要**:本研究利用大肠杆菌表达系统成功表达并纯化CCNA1重组蛋白,优化了诱导条件以提高可溶性蛋白产量,为后续功能研究提供材料。

2. **文献名称**:*Functional Analysis of CCNA1-CDK2 Interaction in Cell Cycle Regulation*

**作者**:Johnson, R., et al.

**摘要**:通过体外重组CCNA1蛋白与CDK2激酶的互作实验,揭示其在细胞周期S期向G2/M期转换中的调控机制,并验证其磷酸化活性。

3. **文献名称**:*Structural Insights into CCNA1 via X-ray Crystallography*

**作者**:Zhang, Y., et al.

**摘要**:解析了CCNA1重组蛋白的晶体结构,发现其N端保守结构域对结合CDK及底物识别至关重要,为靶向药物设计提供依据。

4. **文献名称**:*CCNA1 Overexpression in Leukemia: Role of Recombinant Protein in Apoptosis Assays*

**作者**:Lee, H., et al.

**摘要**:利用重组CCNA1蛋白研究其在急性髓系白血病细胞中的异常表达,证实其过表达可抑制细胞凋亡并促进增殖。

---

如需真实文献,建议通过PubMed或Google Scholar检索关键词“Cyclin A1 recombinant protein”或“CCNA1 expression”获取具体研究。

背景信息

**Background of CCNA1 Recombinant Protein**

CCNA1 (Cyclin A1) is a critical regulatory protein involved in cell cycle progression, primarily functioning during the G2/M phase and meiosis. It belongs to the cyclin family, which partners with cyclin-dependent kinases (CDKs) to control cell cycle checkpoints, ensuring proper DNA replication and mitosis. Unlike the ubiquitously expressed Cyclin A2. CCNA1 exhibits tissue-specific expression, notably in germ cells, hematopoietic cells, and certain cancer types, linking it to reproductive biology and oncogenesis.

Recombinant CCNA1 protein is engineered using genetic cloning techniques, where the *CCNA1* gene is inserted into expression vectors (e.g., bacterial, insect, or mammalian systems) to produce purified protein for research. This recombinant form retains functional domains for CDK binding and substrate recognition, enabling studies on its interaction networks.

Research on CCNA1 has highlighted its dual role: in normal physiology, it supports spermatogenesis and hematopoietic differentiation, while its dysregulation is implicated in leukemias, solid tumors, and chemotherapy resistance. Overexpression of CCNA1 in cancers correlates with poor prognosis, making it a potential therapeutic target or biomarker.

The availability of recombinant CCNA1 facilitates *in vitro* assays (e.g., kinase activity studies), structural analysis, and drug screening. It also aids in deciphering mechanisms underlying cell cycle errors, apoptosis, and cancer progression. Ongoing studies aim to exploit CCNA1's regulatory pathways for novel cancer therapies or diagnostic tools, emphasizing its biomedical relevance.

In summary, CCNA1 recombinant protein serves as a vital tool for exploring cell cycle dynamics, cancer biology, and targeted treatment strategies.

客户数据及评论

折叠内容

大包装询价

×