| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDA2R |
| Uniprot No | Q9HAV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-139aa |
| 氨基酸序列 | ADPMDCQENE YWDQWGRCVT CQRCGPGQEL SKDCGYGEGG DAYCTACPPR RYKSSWGHHR CQSCITCAVI NRVQKVNCTA TSNAVCGDCL PRFYRKTRIG GLQDQECIPC TKQTPTSEVQ CAFQLSLVEA DAPTVPPQEA TLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EDA2R重组蛋白的模拟参考文献示例，基于该领域的典型研究方向：
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1. **标题**：*Structural insights into EDA2R signaling through X-ray crystallography*
**作者**：Schneider, S. et al.
**摘要**：本研究通过X射线晶体学解析了EDA2R受体与其配体EDA-A2的复合物结构，揭示了二者结合的特异性位点，为靶向治疗外胚层发育相关疾病提供了分子基础。
2. **标题**：*Recombinant EDA2R protein rescues ectodermal dysplasia in a murine model*
**作者**：Kumar, P. & Smith, J.L.
**摘要**：在X连锁少汗性外胚层发育不良(XLHED)小鼠模型中，注射重组EDA2R蛋白显著改善了毛发和汗腺发育，证明其潜在治疗价值。
3. **标题**：*Comparative analysis of EDA2R and EDAR signaling pathways in embryonic development*
**作者**：Yan, M. et al.
**摘要**：通过对比EDA2R与EDAR受体的功能差异，研究揭示了二者在外胚层组织发育中的独特作用，并强调EDA2R对特定细胞分化的调控机制。
4. **标题**：*Expression and functional characterization of recombinant human EDA2R in vitro*
**作者**：Rossi, A. & Bianchi, E.
**摘要**：报道了重组人EDA2R蛋白在大肠杆菌中的高效表达与纯化方法，并通过体外实验验证其与配体结合后的下游信号激活能力。
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注：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Google Scholar)检索确认。
The EDA2R (Ectodysplasin A2 Receptor) recombinant protein is a key component in studying signaling pathways related to ectodermal development and associated genetic disorders. EDA2R, also known as XEDAR (X-linked Ectodysplasin-A2 Receptor), belongs to the tumor necrosis factor receptor (TNFR) superfamily. It specifically binds to its ligand EDA-A2. a splice variant of ectodysplasin-A (EDA), to activate downstream NF-κB and JNK signaling cascades. This interaction plays a critical role in embryonic development, particularly in the formation of skin appendages, hair follicles, sweat glands, and teeth.
Mutations in the EDA/EDAR pathway are linked to X-linked hypohidrotic ectodermal dysplasia (XLHED), a genetic disorder characterized by abnormal development of ectodermal tissues. Recombinant EDA2R proteins are engineered to investigate molecular mechanisms underlying these pathologies and to develop therapeutic strategies. Produced through heterologous expression systems (e.g., mammalian cells or E. coli), the recombinant protein typically includes extracellular domains essential for ligand binding, enabling in vitro studies of receptor-ligand interactions.
Research applications include structural analysis of EDA2R-EDA-A2 complexes, screening for small-molecule modulators, and evaluating gene therapy approaches. Its recombinant form allows standardized experimental conditions for comparative studies across disease models. Recent advances in protein engineering have improved solubility and stability, facilitating high-resolution structural studies (e.g., X-ray crystallography) to map binding interfaces. This protein also serves as a tool for diagnosing EDA pathway-related disorders and validating CRISPR/Cas9-edited cellular models. Ongoing studies explore its potential in regenerative medicine for ectodermal tissue repair.
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