纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | MAP4K5 |
Uniprot No | Q9Y4K4 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-846aa |
氨基酸序列 | MEAPLRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVAQPGLSRALAVELLDKVNNPDNHAHYTEADDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGLSSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSEGKTFQLYSHNLIALFEHAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY |
预测分子量 | 95kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP4K5重组蛋白的3篇代表性文献及其摘要概括:
1. **文献名称**:*MAP4K5 regulates AMPK signaling and promotes autophagy in skeletal muscle*
**作者**:Li Y et al.
**摘要**:该研究通过构建MAP4K5重组蛋白,发现其通过激活AMPK/mTOR通路调控骨骼肌细胞自噬,揭示了其在能量代谢和肌肉退行性疾病中的潜在作用。
2. **文献名称**:*Recombinant MAP4K5 kinase domain structure reveals a conserved activation mechanism*
**作者**:Wang X et al.
**摘要**:通过X射线晶体学解析MAP4K5重组蛋白激酶结构域的三维构象,阐明了其催化活性中心的保守性及与底物结合的分子机制。
3. **文献名称**:*MAP4K5 promotes hepatocellular carcinoma progression via ERK-mediated EMT*
**作者**:Chen L et al.
**摘要**:利用重组MAP4K5蛋白进行功能实验,证明其通过ERK信号通路诱导上皮-间质转化(EMT),从而促进肝癌细胞的侵袭和转移。
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**注**:上述文献为示例性内容,实际引用时需核实具体来源(如PubMed ID或DOI)。建议通过关键词“MAP4K5 recombinant protein”在学术数据库检索最新研究。
MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5), also known as Germinal center kinase-like kinase (GLK) or Hematopoietic progenitor kinase/GCK-like kinase (HGK), is a serine/threonine kinase belonging to the mammalian Ste20-like kinase family. It functions upstream in the MAPK (mitogen-activated protein kinase) signaling cascade, which regulates diverse cellular processes, including stress responses, proliferation, differentiation, and apoptosis. Structurally, MAP4K5 contains an N-terminal catalytic kinase domain, a central coiled-coil domain for protein interactions, and a C-terminal regulatory domain. Its activation typically occurs through phosphorylation, often triggered by cellular stressors like oxidative stress, cytokines, or nutrient deprivation.
MAP4K5 plays critical roles in metabolic regulation and immune-inflammatory pathways. It interacts with key signaling components, such as the TAK1 (TGF-β-activated kinase 1) complex, to modulate pathways like JNK/p38 MAPK and NF-κB. Studies highlight its involvement in insulin resistance, obesity, and type 2 diabetes by regulating glucose uptake and lipid metabolism through crosstalk with mTORC1 and AMPK pathways. Additionally, MAP4K5 influences immune cell activation and inflammatory responses, linking it to autoimmune diseases and cancer progression. Dysregulation of MAP4K5 has been implicated in neurodegenerative disorders and cardiovascular diseases, underscoring its broad pathophysiological relevance.
Recombinant MAP4K5 protein is produced using expression systems like E. coli or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. It serves as a vital tool for in vitro kinase assays, substrate identification, and inhibitor screening in drug discovery. Researchers utilize it to dissect MAPK signaling mechanisms, validate therapeutic targets, and model disease-associated kinase activity. Its commercial availability accelerates studies aiming to modulate MAP4K5-driven pathways for therapeutic interventions.
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