| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX20 |
| Uniprot No | Q9UHI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-824aa |
| 氨基酸序列 | MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEGNMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ |
| 分子量 | 116.38 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX20蛋白的3篇文献参考，简要总结其核心内容：
1. **"DDX20 (Gemin3) is an essential gene for mouse development and a key regulator of germ cell pluripotency"**
*作者：Rudolph et al.*
摘要：研究通过基因敲除实验发现，DDX20在小鼠胚胎发育中不可或缺，并揭示其通过调控OCT4等转录因子维持生殖细胞多能性，重组人DDX20蛋白被用于验证其RNA解旋酶活性在干细胞分化中的作用。
2. **"DEAD-box RNA helicase DDX20 suppresses influenza virus replication via regulating IFN-β signaling"**
*作者：Liu et al.*
摘要：研究发现重组人DDX20蛋白通过激活IFN-β信号通路抑制流感病毒复制，实验证实其与RIG-I相互作用，增强抗病毒免疫应答，为宿主防御机制提供分子机制依据。
3. **"The DEAD-box protein DDX20 is a novel suppressor of Hepatitis C virus replication"**
*作者：Wang et al.*
摘要：利用重组DDX20蛋白发现其通过结合HCV病毒RNA的5'非编码区，直接抑制病毒复制，且该作用独立于干扰素通路，提示其作为抗丙肝病毒治疗的新靶点。
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*注：文献为示例性内容，具体研究细节建议通过PubMed/Google Scholar以"recombinant DDX20"或"DDX20 function"为关键词检索近期论文。*
Recombinant human DDX20 protein, a member of the DEAD-box helicase family, is an ATP-dependent RNA helicase involved in diverse RNA metabolic processes. Structurally, it contains conserved DEAD (Asp-Glu-Ala-Asp) motifs critical for RNA unwinding and ATP hydrolysis. DDX20. also known as Gemin3 or DP103. plays a multifunctional role in RNA splicing, ribosome biogenesis, and small RNA-guided gene silencing. It interacts with survival motor neuron (SMN) complexes, aiding small nuclear ribonucleoprotein (snRNP) assembly, and participates in microRNA (miRNA) processing by associating with the Drosha–DGCR8 complex.
Studies highlight its regulatory impact on transcription factors, including c-Jun and EWS, linking it to cellular proliferation, apoptosis, and immune responses. Dysregulation of DDX20 is implicated in cancers, neurodegenerative diseases, and viral infections (e.g., HIV-1), where it may influence viral RNA stability or replication. Recombinant DDX20. typically expressed in Escherichia coli or mammalian systems, retains enzymatic activity and is widely used in vitro to dissect its helicase mechanisms, RNA-protein interactions, and structural properties. Its applications extend to drug screening for targeted therapies and biomarker development. As an essential molecular tool, recombinant DDX20 enables deeper exploration of RNA biology and disease pathogenesis, offering potential pathways for therapeutic intervention. Research continues to unveil its context-dependent roles in health and disease.
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