| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX10 |
| Uniprot No | Q13206 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-875aa |
| 氨基酸序列 | MGKTANSPGSGARPDPVRSFNRWKKKHSHRQNKKKQLRKQLKKPEWQVERESISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLLQKKVPVKEIKINPEKLIDVQKKLESILAQDQDLKERAQRCFVSYVRSVYLMKDKEVFDVSKLPIPEYALSLGLAVAPRVRFLQKMQKQPTKELVRSQADKVIEPRAPSLTNDEVEEFRAYFNEKMSILQKGGKRLEGTEHRQDNDTGNEEQEEEEDDEEEMEEKLAKAKGSQAPSLPNTSEAQKIKEVPTQFLDRDEEEEDADFLKVKRHNVFGLDLKDEKTLQKKEPSKSSIKKKMTKVAEAKKVMKRNFKVNKKITFTDEGELVQQWPQMQKSAIKDAEEDDDTGGINLHKAKERLQEEDKFDKEEYRKKIKAKHREKRLKEREARREANKRQAKAKDEEEAFLDWSDDDDDDDDGFDPSTLPDPDKYRSSEDSDSEDMENKISDTKKKQGMKKRSNSEVEDVGPTSHNRKKARWDTLEPLDTGLSLAEDEELVLHLLRSQS |
| 分子量 | 127.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX10蛋白的模拟参考文献示例，涵盖其功能、疾病关联及结构研究：
1. **"Functional characterization of recombinant human DDX10 protein in RNA helicase activity" by A. Takahashi et al.**
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组人DDX10蛋白，通过生化和酶学分析证实其具有ATP依赖的RNA解旋酶活性，揭示了其在RNA代谢(如剪接或核糖体生物合成)中的潜在作用。
2. **"Overexpression of DDX10 in acute myeloid leukemia: implications for leukemogenesis" by L. Wang et al.**
**摘要**：研究发现，DDX10在急性髓系白血病(AML)患者样本中显著高表达，并证明其通过调控细胞周期相关RNA的代谢促进白血病细胞异常增殖，提示其可作为AML治疗的潜在靶点。
3. **"Structural insights into the DEAD-box protein DDX10: Implications for RNA binding and ATP hydrolysis" by H. Müller et al.**
**摘要**：利用X射线晶体学解析了DDX蛋白的结构，重点揭示了其ATP结合域和RNA结合基序的构象变化，为理解DEAD-box家族蛋白的RNA解旋机制提供了结构基础。
4. **"DDX10 interacts with HCV NS5B and facilitates viral RNA replication" by K. Suzuki et al.**
**摘要**：研究显示，重组人DDX10蛋白与丙型肝炎病毒(HCV)的NS5B聚合酶直接相互作用，通过促进病毒RNA二级结构的解旋来增强病毒复制效率，为抗病毒策略提供了新方向。
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*注：以上文献为示例性质，实际研究中建议通过学术数据库(如PubMed、Web of Science)检索最新及权威文献。*
Recombinant human DDX10 protein is a biologically engineered form of the DDX10 (DEAD-box helicase 10) protein, a member of the DEAD-box RNA helicase family. These proteins are characterized by the conserved "DEAD" motif (Asp-Glu-Ala-Asp), which is critical for ATP-dependent RNA helicase activity. DDX10 plays essential roles in RNA metabolism, including ribosome biogenesis, pre-mRNA splicing, and RNA transport. It localizes primarily to the nucleolus and participates in the processing and maturation of ribosomal RNA (rRNA), contributing to the assembly of ribosomal subunits. Dysregulation of DDX10 has been implicated in various diseases, particularly cancer. For instance, chromosomal translocations involving DDX10 have been identified in certain leukemias, leading to oncogenic fusion proteins. Recombinant DDX10 is typically produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable functional studies. Its production allows researchers to investigate DDX10's molecular mechanisms, enzymatic properties (e.g., ATPase/helicase activity), and interactions with nucleic acids or partner proteins. This recombinant tool also supports drug discovery efforts targeting helicase-driven pathologies, including cancer and viral infections exploiting host RNA machinery. Structural studies using recombinant DDX10 help elucidate its conserved domains and guide therapeutic interventions.
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