| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDHD2 |
| Uniprot No | O94830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MSSVQSQQEQLSQSDPSPSPNSCSSFELIDMDAGSLYEPVSPHWFYCKIIDSKETWIPFNSEDSQQLEEAYSSGKGCNGRVVPTDGGRYDVHLGERMRYAVYWDELASEVRRCTWFYKGDKDNKYVPYSESFSQVLEETYMLAVTLDEWKKKLESPNREIIILHNPKLMVHYQPVAGSDDWGSTPTEQGRPRTVKRGVENISVDIHCGEPLQIDHLVFVVHGIGPACDLRFRSIVQCVNDFRSVSLNLLQTHFKKAQENQQIGRVEFLPVNWHSPLHSTGVDVDLQRITLPSINRLRHFTNDTILDVFFYNSPTYCQTIVDTVASEMNRIYTLFLQRNPDFKGGVSIAGHSLGSLILFDILTNQKDSLGDIDSEKDSLNIVMDQGDTPTLEEDLKKLQLSEFFDIFEKEKVDKEALALCTDRDLQEIGIPLGPRKKILNYFSTRKNSMGIKRPAPQPASGANIPKESEFCSSSNTRNGDYLDVGIGQVSVKYPRLIYKPEIFFAFGSPIGMFLTVRGLKRIDPNYRFPTCKGFFNIYHPFDPVAYRIEPMVVPGVEFEPMLIPHHKGRKRMHLELREGLTRMSMDLKNNLLGSLRMAWKSFTRAPYPALQASETPEETEAEPESTSEKPSDVNTEETSVAVKEEVLPINVGMLNGGQRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTVLLVLKEIYQTQGIFLDQPLQ |
| 分子量 | 107.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDHD2蛋白的3篇模拟参考文献示例(信息为模拟生成，非真实文献)：
1. **文献名称**：*DDHD2 mutations alter cellular phospholipid metabolism linked to hereditary spastic paraplegia*
**作者**：Kirsten Jahn et al.
**摘要**：研究揭示了DDHD2基因突变导致磷脂酶活性丧失，影响神经细胞磷脂代谢，引发SPG54型痉挛性截瘫的分子机制。通过重组人DDHD2蛋白体外实验证实突变体无法水解特定磷脂底物。
2. **文献名称**：*Structural and functional analysis of human DDHD2 in membrane-associated processes*
**作者**：Andrea Diederich et al.
**摘要**：解析了重组人DDHD2蛋白的晶体结构，发现其依赖SAM结构域调控内质网膜形态，并证明其磷脂酶活性依赖于二价金属离子(如Ca²⁺)。
3. **文献名称**：*Recombinant human DDHD2 rescues mitochondrial defects in cellular models of SPG54*
**作者**：Thomas Burgert et al.
**摘要**：通过体外表达纯化的重组人DDHD2蛋白，成功恢复了SPG54患者来源细胞中线粒体形态异常和脂质堆积表型，为基因治疗提供理论基础。
(注：以上内容为示例，实际文献需通过学术数据库检索获取。)
DDHD2 (DDHD domain-containing protein 2) is a mammalian intracellular phospholipase belonging to the DDHD protein family, characterized by a conserved DDHD domain that facilitates catalytic activity. It hydrolyzes phospholipids such as phosphatidic acid (PA) and phosphatidylinositol (PI), regulating lipid metabolism and membrane trafficking. The gene encoding human DDHD2 is associated with autosomal recessive hereditary spastic paraplegia type 54 (SPG54), a neurodegenerative disorder characterized by corticospinal tract degeneration, intellectual disability, and lipid droplet accumulation in cells.
Recombinant human DDHD2 protein is engineered using expression systems like E. coli or insect cells, enabling large-scale production for functional studies. Its purification typically involves affinity chromatography tags (e.g., His-tag) followed by enzymatic activity validation. Studies using recombinant DDHD2 have elucidated its role in lipid homeostasis, Golgi organization, and autophagosome-lysosome fusion. Mutations disrupting its phospholipase activity are linked to SPG54 pathogenesis, underscoring its importance in neuronal health. Recombinant DDHD2 also serves as a tool to screen therapeutic compounds targeting lipid metabolism disorders or neurodegenerative diseases. Current research focuses on its interplay with other lipid-modifying enzymes and its potential regulatory mechanisms in cellular stress responses.
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