| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDHD1 |
| Uniprot No | Q8NEL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-872aa |
| 氨基酸序列 | MNYPGRGSPRSPEHNGRGGGGGAWELGSDARPAFGGGVCCFEHLPGGDPDDGDVPLALLRGEPGLHLAPGTDDHNHHLALDPCLSDENYDFSSAESGSSLRYYSEGESGGGGSSLSLHPPQQPPLVPTNSGGGGATGGSPGERKRTRLGGPAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGARPQGGDRDGDHVCSPTGPASSSGEDDDEDRACGFCQSTTGHEPEMVELVNIEPVCVRGGLYEVDVTQGECYPVYWNQADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLNCFRGQQMQENFDIEVSKSIDGKDAVHSFKLSRNHVDWHSVDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTTHIVFVVHGIGQKMDQGRIIKNTAMMREAARKIEERHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMTGWNPVRLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLKEIEERLHGLKASSMTQTPALKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKRYSNISPVQIHWYNTSNPLPYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSSTTQSSETSKDSMEDEKKPVASPSATTVGTQTLPHSSSGFLDSALELDHRIDFELREGLVESRYWSAVTSHTAYWSSLDVALFLLTFMYKHEHDDDAKPNLDPI |
| 分子量 | 123.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDHD1蛋白的3篇参考文献示例(虚构内容，供参考)：
1. **文献名称**：*Structural insights into human DDHD1 phospholipase domain by X-ray crystallography*
**作者**：Inoue H. et al. (2015)
**摘要**：首次解析了重组人DDHD1蛋白催化结构域的晶体结构，揭示了其磷脂酶活性位点的关键氨基酸组成及底物结合机制。
2. **文献名称**：*DDHD1 deficiency impairs lipid metabolism and causes hereditary spastic paraplegia*
**作者**：Inloes J.M. et al. (2018)
**摘要**：通过重组人DDHD1蛋白的功能实验，发现其脂水解活性缺失导致细胞内磷脂代谢异常，与遗传性痉挛性截瘫(SPG28)相关。
3. **文献名称**：*Recombinant DDHD1 expression in HEK293 cells: a model for enzymatic characterization*
**作者**：Ishikawa T. et al. (2020)
**摘要**：建立高效表达重组人DDHD1蛋白的哺乳细胞系统，验证其磷脂酶A1活性，并开发了基于荧光底物的酶活检测方法。
(注：以上文献及内容为模拟，实际研究请参考数据库如PubMed。)
DDHD1 (DDHD domain-containing protein 1) is a mammalian intracellular phospholipase that belongs to the phospholipase A1 (PLA1) family. It catalyzes the hydrolysis of phospholipids at the sn-1 position, regulating lipid metabolism and signaling pathways. The protein is widely expressed in tissues, particularly in the brain, and is localized to the Golgi apparatus and cytoplasm. Structurally, DDHD1 contains a conserved DDHD domain, which harbors phospholipase activity, and a sterile alpha motif (SAM) involved in protein interactions.
Recombinant human DDHD1 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and functional studies. Research has linked DDHD1 to lipid homeostasis, membrane trafficking, and autophagosome formation. Mutations in the DDHD1 gene are associated with hereditary spastic paraplegia (SPG28), a neurodegenerative disorder characterized by progressive lower limb spasticity. Recombinant DDHD1 is widely used to investigate its enzymatic mechanisms, lipid-binding properties, and roles in neurodevelopment and disease. Current studies also explore its potential as a therapeutic target for lipid metabolism-related disorders.
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