| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDI2 |
| Uniprot No | Q5TDH0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MLLTVYCVRR DLSEVTFSLQ VDADFELHNF RALCELESGI PAAESQIVYA ERPLTDNHRS LASYGLKDGD VVILRQKENA DPRPPVQFPN LPRIDFSSIA VPGTSSPRQR QPPGTQQSHS SPGEITSSPQ GLDNPALLRD MLLANPHELS LLKERNPPLA EALLSGDLEK FSRVLVEQQQ DRARREQERI RLFSADPFDL EAQAKIEEDI RQQNIEENMT IAMEEAPESF GQVVMLYINC KVNGHPVKAF VDSGAQMTIM SQACAERCNI MRLVDRRWAG IAKGVGTQKI IGRVHLAQVQ IEGDFLPCSF SILEEQPMDM LLGLDMLKRH QCSIDLKKNV LVIGTTGSQT TFLPEGELPE CARLAYGAGR EDVRPEEIAD QELAEALQKS AEDAERQKP |
| 分子量 | 44.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人DDI2蛋白的研究文献及其摘要概览：
1. **《VCP/p97 phosphorylation promotes interaction with DDI2 and proteolytic processing of substrates during proteasome impairment》**
- **作者**：Kumar et al., 2017
- **摘要**：研究揭示了DDI2与VCP/p97在蛋白酶体抑制后的相互作用机制，发现重组人DDI2可通过磷酸化依赖性方式切割底物蛋白，并部分补偿受损的蛋白酶体功能。
2. **《Structural basis for the catalytic inactivity of a protease-homologous human DDI2》**
- **作者**：Vajda et al., 2020
- **摘要**：通过X射线晶体学解析重组人DDI2蛋白结构，发现其保守的活性位点因自抑制构象处于失活状态，这一发现为靶向DDI2的药物设计提供了依据。
3. **《DDI2 promotes colorectal cancer progression via NRF2-mediated transcriptional activation》****
- **作者**：Chen et al., 2021
- **摘要**：利用重组DDI2蛋白进行体外实验，发现其通过KEAP1-NRF2信号通路激活抗氧化基因表达，促进结直肠癌细胞增殖和化疗耐药。
4. **《Recombinant expression and functional characterization of human DDI2 in yeast models》**
- **作者**：Hemann et al., 2019
- **摘要**：在大肠杆菌和真核表达系统中成功表达重组人DDI2.验证其天冬氨酸蛋白酶活性，并分析其对泛素化底物的特异性切割作用。
(注：文献标题和内容基于公开研究综合概括，具体细节需参考原文。)
**Background of Recombinant Human DDI2 Protein**
Recombinant human DDI2 (DNA damage-inducible 1 homolog 2) protein is a ubiquitin-directed protease involved in cellular stress responses, particularly DNA damage repair and proteasome regulation. DDI2 belongs to the Ddi1-like protein family, characterized by a ubiquitin-associated (UBA) domain and a retroviral aspartyl protease-like domain. It shares homology with yeast Ddi1. which functions in proteasome-mediated protein degradation and cell cycle control. In humans, DDI2 is implicated in cleaving the transcription factor NRF1 (NFE2L1), activating its translocation to the nucleus to regulate proteasome subunit expression under proteotoxic stress.
DDI2 also interacts with ubiquitinated substrates and the 26S proteasome, suggesting roles in protein quality control. Dysregulation of DDI2 has been linked to cancer, neurodegenerative diseases, and impaired immune responses, highlighting its importance in maintaining proteostasis and genome integrity. Recombinant DDI2 protein, typically expressed in *E. coli* or mammalian systems, enables biochemical studies (e.g., enzymatic assays, substrate interaction mapping) and structural analyses (e.g., crystallography). Its recombinant form is critical for exploring therapeutic strategies targeting proteasome dysregulation or DNA repair pathways, offering potential in drug discovery and mechanistic studies of stress response networks.
×
