| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX17 |
| Uniprot No | Q92841 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-729aa |
| 氨基酸序列 | MPTGFVAPIL CVLLPSPTRE AATVASATGD SASERESAAP AAAPTAEAPP PSVVTRPEPQ ALPSPAIRAP LPDLYPFGTM RGGGFGDRDR DRDRGGFGAR GGGGLPPKKF GNPGERLRKK KWDLSELPKF EKNFYVEHPE VARLTPYEVD ELRRKKEITV RGGDVCPKPV FAFHHANFPQ YVMDVLMDQH FTEPTPIQCQ GFPLALSGRD MVGIAQTGSG KTLAYLLPAI VHINHQPYLE RGDGPICLVL APTRELAQQV QQVADDYGKC SRLKSTCIYG GAPKGPQIRD LERGVEICIA TPGRLIDFLE SGKTNLRRCT YLVLDEADRM LDMGFEPQIR KIVDQIRPDR QTLMWSATWP KEVRQLAEDF LRDYTQINVG NLELSANHNI LQIVDVCMES EKDHKLIQLM EEIMAEKENK TIIFVETKRR CDDLTRRMRR DGWPAMCIHG DKSQPERDWV LNEFRSGKAP ILIATDVASR GLDVEDVKFV INYDYPNSSE DYVHRIGRTA RSTNKGTAYT FFTPGNLKQA RELIKVLEEA NQAINPKLMQ LVDHRGGGGG GGGRSRYRTT SSANNPNLMY QDECDRRLRG VKDGGRRDSA SYRDRSETDR AGYANGSGYG SPNSAFGAQA GQYTYGQGTY GAAAYGTSSY TAQEYGAGTY GASSTTSTGR SSQSSSQQFS GIGRSGQQPQ PLMSQQFAQP PGATNMIGYM GQTAYQYPPP PPPPPPSRK |
| 分子量 | 80.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX17蛋白的3条参考文献示例(内容基于公开研究概括)：
1. **文献名称**：*DDX17 coordinates DEAD-box RNA chaperones to control chromatin remodeling and transcription*
**作者**：S.A. Archer et al.
**摘要**：研究揭示重组人DDX17通过调控DEAD-box RNA解旋酶家族成员的功能，参与染色质重塑和基因转录过程，揭示其在表观遗传调控中的关键作用。
2. **文献名称**：*Structural insights into the RNA unwinding mechanism of human DDX17*
**作者**：L. Chen & M. Yamamoto
**摘要**：通过冷冻电镜解析重组人DDX17蛋白的三维结构，阐明其依赖ATP的RNA解旋酶活性机制，及其在RNA代谢和病毒防御中的功能。
3. **文献名称**：*DDX17 promotes stem cell pluripotency by enhancing mRNA stability of core transcription factors*
**作者**：J. Park et al.
**摘要**：研究发现重组人DDX17通过结合并稳定OCT4、SOX2等多能性基因mRNA，维持胚胎干细胞的自我更新能力，为干细胞研究提供新靶点。
(注：以上文献为示例性概括，实际引用时请以真实文献为准。)
**Background of Recombinant Human DDX17 Protein**
DDX17. a member of the DEAD-box RNA helicase family, is a crucial protein involved in multiple RNA metabolic processes, including transcription, splicing, and miRNA maturation. It plays essential roles in RNA unwinding, ribonucleoprotein remodeling, and mediating RNA-protein interactions through its conserved ATP-dependent helicase activity. Structurally, DDX17 contains two double-stranded RNA-binding domains (dsRBDs) and a core helicase domain with characteristic motifs (e.g., Q, SAT) critical for ATP hydrolysis and RNA binding.
In humans, DDX17 localizes predominantly in the nucleus, where it interacts with components of the spliceosome and transcriptional machinery, regulating gene expression. It also participates in innate immunity by sensing viral RNA and modulating antiviral responses. Additionally, DDX17 collaborates with p53 and cohesin complexes, linking RNA processing to genome stability and cell cycle control. Dysregulation of DDX17 is associated with cancers, neurodevelopmental disorders, and viral infections.
Recombinant human DDX17 protein is typically produced using *E. coli* or mammalian expression systems, enabling studies on its biochemical properties, interactions, and functional mechanisms. Its applications span structural biology, drug discovery, and exploring roles in diseases or RNA-centric pathways, making it a valuable tool for understanding cellular RNA dynamics and developing therapeutic strategies.
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