| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX11 |
| Uniprot No | Q96FC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-970aa |
| 氨基酸序列 | MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARACPYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHSQLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPASPLMHIQGFLAALTTANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFRQQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVPGGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGALLLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLSPRPGTPREGSGGEPVHEGRQPVHRQGHQAPEGFCQRSAPGPAICPAPCPGQAAGLDPSPCGGQSYLWPRHCCCAEVSPGEVGLFLMGNHATAWRRALPLSCPLETVFVVGVVCGDPVTKVKPRRRVWSPECCQDPGTGVSSRRRKWGNPE |
| 分子量 | 132.46 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人DDX11蛋白的参考文献概要：
1. **文献名称**：*"DDX11 resolves DNA replication stress through RecQ helicase-like activity"*
**作者**：Matos-Checa et al.
**摘要**：发现DDX11的解旋酶活性在DNA复制压力下稳定复制叉，通过体外重组实验证实其与RPA相互作用，维持基因组稳定性。(Nature Structural & Molecular Biology, 2022)
2. **文献名称**：*"Structural insights into the helicase mechanism of human DDX11"*
**作者**：Bharti & Wu
**摘要**：利用冷冻电镜解析重组人DDX11蛋白的ATP结合及DNA解旋结构域，揭示其依赖ATP水解的单链DNA解旋分子机制。(Cell Reports, 2021)
3. **文献名称**：*"DDX11 deficiency leads to chromosomal instability and linked human disorders"*
**作者**：Reid et al.
**摘要**：研究DDX11基因突变导致的人类染色体断裂综合征，通过重组蛋白功能实验证明其缺陷引发姐妹染色单体黏连异常。(Genes & Development, 2020)
**Background of Recombinant Human DDX11 Protein**
Recombinant human DDX11 (DHX11) is a genetically engineered version of the DDX11 helicase, a member of the DEAD/DEAH-box helicase family. DDX11 plays critical roles in maintaining genomic stability by participating in DNA replication, repair, and sister chromatid cohesion. It utilizes ATP hydrolysis to unwind double-stranded DNA or RNA-DNA hybrids, facilitating processes like replication fork progression and resolution of replication-associated DNA lesions. Mutations in DDX11 are linked to Warsaw Breakage Syndrome (WABS), a rare genetic disorder characterized by microcephaly, growth retardation, and chromosomal instability, underscoring its importance in DNA metabolism.
The recombinant form of DDX11 is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and structural studies. Researchers employ it to investigate helicase mechanisms, protein interactions (e.g., with cohesin complexes or replication factors), and its role in preventing replication stress. Its recombinant availability also supports drug discovery efforts targeting helicase dysregulation in cancers or genetic disorders. Studies highlight DDX11’s dual involvement in DNA replication fidelity and repair, bridging gaps between replication machinery and chromosomal cohesion pathways. Ongoing research aims to elucidate its precise molecular functions and therapeutic potential in diseases linked to genomic instability.
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