| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX18 |
| Uniprot No | Q9NVP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670aa |
| 氨基酸序列 | MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMTRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH |
| 分子量 | 101.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX18蛋白的模拟参考文献示例(仅供学习参考，实际文献需通过学术数据库检索)：
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1. **文献名称**: *"Recombinant DDX18 promotes colorectal cancer progression by enhancing ribosome biogenesis"*
**作者**: Zhou, J., et al.
**摘要**: 该研究通过体外表达重组人DDX18蛋白，发现其过表达在结直肠癌细胞中显著促进核糖体RNA(rRNA)的加工，并加速细胞增殖。实验表明，DDX18的ATP酶活性缺失突变体(K123A)可阻断这一效应，提示其依赖酶活性调控肿瘤生长。
2. **文献名称**: *"Host factor DDX18 facilitates influenza A virus replication via interaction with viral NP protein"*
**作者**: Zhang, Y., et al.
**摘要**: 本研究发现重组人DDX18蛋白可与甲型流感病毒的核蛋白(NP)直接结合，通过siRNA敲低DDX18后，病毒复制效率显著下降;而外源回补重组DDX18可恢复病毒复制能力，表明其作为宿主因子在病毒生命周期中起关键作用。
3. **文献名称**: *"Crystal structure of human DDX18 reveals a conserved DEAD-box helicase architecture"*
**作者**: Chen, L., et al.
**摘要**: 本研究解析了重组人DDX18蛋白的晶体结构(分辨率2.8 Å)，揭示了其N端结构域对RNA底物识别的重要性，并基于突变实验验证了核心结构域(Motif I-III)在ATP水解和RNA解旋中的功能。
4. **文献名称**: *"DDX18 coordinates rRNA processing and cell cycle progression in human cells"*
**作者**: Wang, Q., et al.
**摘要**: 利用重组DDX18蛋白进行体外RNA结合实验，发现其特异性结合28S rRNA前体区域;细胞实验表明，DDX18通过调控rRNA成熟影响G1/S期转换，揭示了其在细胞周期与核糖体合成间的分子联系。
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**注意**：以上内容为模拟生成的示例，实际研究中请通过PubMed、Web of Science或Google Scholar检索具体文献，并参考期刊的官方引用格式。
Human DDX18 is a member of the DEAD-box RNA helicase family, characterized by a conserved DEAD (Asp-Glu-Ala-Asp) motif. These ATP-dependent enzymes play critical roles in RNA metabolism, including unwinding RNA secondary structures, remodeling RNA-protein complexes, and coordinating diverse cellular processes like ribosome biogenesis, transcription, splicing, and stress responses. DDX18 localizes predominantly to the nucleolus and is involved in ribosomal RNA (rRNA) processing and ribosome assembly, ensuring proper protein synthesis. It also participates in DNA damage repair, cell cycle regulation, and viral RNA sensing, linking it to genome stability and innate immunity.
Recombinant human DDX18 protein is produced via genetic engineering in bacterial or eukaryotic expression systems, enabling controlled study of its biochemical activities. Its purified form retains ATPase and helicase functions, facilitating in vitro experiments to dissect molecular mechanisms. Researchers use it to explore interactions with RNA/DNA substrates, cofactors (e.g., non-structural proteins in viral replication), or small-molecule inhibitors. Dysregulation of DDX18 is implicated in cancers, neurodevelopmental disorders, and viral infections, making it a target for therapeutic development. Structural studies of recombinant DDX18. often fused with tags like His or GST for purification, further elucidate its domain architecture and conformational dynamics during catalysis. This tool bridges functional insights into cellular homeostasis and disease pathways.
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