| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX42 |
| Uniprot No | Q86XP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-819aa |
| 氨基酸序列 | MAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS |
| 分子量 | 116.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX42蛋白的3篇代表性文献的简要列表(注：以下内容为示例，实际文献需根据具体研究查询数据库)：
1. **文献名称**：*DDX42 regulates apoptosis and proliferation of colorectal cancer cells through the p53 signaling pathway*
**作者**：Wang Y, Li T, et al.
**摘要**：研究通过重组人DDX42蛋白体外表达，发现其通过与p53蛋白相互作用调控结直肠癌细胞凋亡与增殖，提示DDX42在癌症中的潜在治疗靶点作用。
2. **文献名称**：*Structural and functional analysis of the DEAD-box helicase DDX42*
**作者**：Garcia D, Linder P, et al.
**摘要**：解析了重组人DDX42蛋白的晶体结构，揭示其ATP酶和解旋酶活性的分子机制，并证明其在RNA代谢及DNA损伤修复中的关键功能。
3. **文献名称**：*DDX42 interacts with viral RNA to inhibit SARS-CoV-2 replication*
**作者**：Chen X, Zhou J, et al.
**摘要**：利用重组DDX42蛋白研究发现其能够直接结合新冠病毒RNA，抑制病毒复制，为抗病毒免疫应答机制提供了新见解。
**注意**：以上文献信息为示例性质，实际引用时需通过PubMed、Web of Science等平台核实具体论文标题、作者及摘要内容。
**Background of Recombinant Human DDX42 Protein**
The DEAD-box helicase DDX42 is a member of the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif, which enables ATP-dependent RNA or DNA unwinding. DDX42 plays versatile roles in RNA metabolism, including transcription, splicing, RNA editing, and ribosome biogenesis. It also participates in DNA damage repair and innate immune responses by interacting with viral RNAs or regulating antiviral signaling pathways. Dysregulation of DDX42 has been linked to cancers, neurodegenerative disorders, and viral infections, underscoring its importance in cellular homeostasis.
Recombinant human DDX42 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functionally active form. This recombinant tool facilitates *in vitro* studies on DDX42's enzymatic activities, structural features, and molecular interactions. Researchers employ it to dissect its roles in genome stability, stress responses, and virus-host interactions. Additionally, it serves as a potential therapeutic target for drug discovery, particularly in diseases associated with RNA processing defects or aberrant helicase activity. Its application spans basic research, diagnostics, and targeted therapy development.
×
