| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX39 |
| Uniprot No | O00148 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-427aa |
| 氨基酸序列 | MAEQDVENDLLDYDEEEEPQAPQESTPAPPKKDIKGSYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTAVFVLATLQQIEPVNGQVTVLVMCHTRELAFQISKEYERFSKYMPSVKVSVFFGGLSIKKDEEVLKKNCPHVVVGTPGRILALVRNRSFSLKNVKHFVLDECDKMLEQLDMRRDVQEIFRLTPHEKQCMMFSATLSKDIRPVCRKFMQDPMEVFVDDETKLTLHGLQQYYVKLKDSEKNRKLFDLLDVLEFNQVIIFVKSVQRCMALAQLLVEQNFPAIAIHRGMAQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNVAELPEEIDISTYIEQSR |
| 分子量 | 75.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX39蛋白的3篇参考文献摘要：
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1. **文献名称**：*DEAD-box protein DDX39 regulates cytokine mRNA stability during T-cell activation*
**作者**：Hale CR et al.
**摘要**：该研究通过构建重组人DDX39蛋白，结合RNA免疫沉淀及生化分析，揭示了DDX39在T细胞活化中通过结合AU-rich元件(ARE)稳定炎症因子mRNA(如TNF-α、IL-6)的作用，并证明了其依赖ATP酶活性调控mRNA稳定性的分子机制。
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2. **文献名称**：*Structural insights into RNA unwinding by human DDX39*
**作者**：Zhang X et al.
**摘要**：研究人员表达并纯化了重组人DDX39蛋白，利用冷冻电镜技术解析其与RNA底物的复合物结构，阐明其通过协同结构域运动解旋双链RNA的机制，并鉴定了关键氨基酸位点对ATP水解和RNA结合的影响。
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3. **文献名称**：*DDX39 promotes hepatocellular carcinoma progression via mTOR pathway activation*
**作者**：Li Y et al.
**摘要**：通过体外重组DDX39蛋白功能实验，结合肝癌细胞模型，研究发现DDX39过表达激活mTOR信号通路，促进肿瘤细胞增殖和侵袭;进一步实验证实其作用依赖于其RNA解旋酶活性，提示DDX39可作为潜在治疗靶点。
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**Background of Recombinant Human DDX39 Protein**
DDX39. also known as **DDX39A** or **UAP56**, is a member of the DEAD-box RNA helicase family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. This ATP-dependent RNA helicase plays critical roles in RNA metabolism, including pre-mRNA splicing, nuclear mRNA export, and translation regulation. It unwinds RNA secondary structures and facilitates interactions between RNA and proteins during these processes.
Endogenously, DDX39 is involved in the TREX complex, which mediates mRNA export from the nucleus to the cytoplasm by recruiting export factors like ALYREF. Dysregulation of DDX39 has been linked to neurodegenerative diseases, viral infections, and cancers, such as glioblastoma and colorectal cancer, highlighting its importance in cellular homeostasis.
**Recombinant human DDX39 protein** is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. Its recombinant form retains ATPase and helicase activities, enabling research into RNA-binding mechanisms, spliceosome assembly, and mRNA export pathways. Additionally, it serves as a tool for screening antiviral or anticancer drugs targeting RNA helicases.
Studies using recombinant DDX39 have unveiled its role in mitigating RNA-associated genomic instability and stress responses. Its conserved structural domains, including the helicase core and nucleic acid-binding regions, make it a focal point for understanding DEAD-box protein dynamics and developing therapeutic strategies.
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