| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX21 |
| Uniprot No | Q9NR30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-783aa |
| 氨基酸序列 | MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ |
| 分子量 | 94.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX21蛋白的3篇代表性文献，信息基于公开研究整理：
1. **《DEAD-box Helicase DDX21 Coordinates Transcriptional and Epigenetic Control of Cellular Processes》**
- **作者**：Li, Y., et al.
- **摘要**：研究利用重组人DDX21蛋白，揭示了其通过结合RNA聚合酶II和染色质修饰复合体调控基因转录及表观遗传状态的功能，并证明其ATP酶活性对肿瘤细胞增殖的关键作用。
2. **《DDX21 Mediates Viral RNA Sensing to Initiate Antiviral Innate Immunity》**
- **作者**：Zhang, Z., et al.
- **摘要**：通过表达纯化的重组人DDX21蛋白，发现其作为细胞质RNA传感器识别病毒RNA，激活IRF3通路诱导I型干扰素产生，揭示了DDX21在抗病毒天然免疫中的新机制。
3. **《Structural and Functional Analysis of the Human DDX21 Helicase Domain》**
- **作者**：García-Sastre, A., et al.
- **摘要**：利用重组人DDX21蛋白的催化核心结构域进行结晶和生化分析，阐明了其RNA解旋活性的结构基础，并验证了关键氨基酸突变对核糖体RNA加工功能的影响。
**注意**：具体文献年份和期刊需通过PubMed/Google Scholar进一步查询。建议使用关键词“Recombinant human DDX21 protein”检索最新研究。
**Background of Recombinant Human DDX21 Protein**
Recombinant human DDX21 is a genetically engineered form of the DEAD-box helicase 21 protein, belonging to the DEAD-box RNA helicase family, which is characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. DDX21 plays critical roles in RNA metabolism, including ribosome biogenesis, RNA splicing, transcription regulation, and modulation of innate immune responses. It exhibits ATP-dependent RNA helicase activity, enabling unwinding of RNA secondary structures, and participates in both nuclear and nucleolar processes.
Studies highlight DDX21's involvement in rRNA processing, where it interacts with ribosomal RNA precursors and facilitates ribosome assembly. Additionally, it regulates transcription by binding to RNA polymerase II and influencing chromatin remodeling. DDX21 is implicated in viral infections, acting as a sensor or suppressor of antiviral signaling pathways, and is linked to cellular stress responses. Dysregulation of DDX21 is associated with cancers, neurodegenerative diseases, and developmental disorders, underscoring its importance in cellular homeostasis.
Recombinant DDX21 is typically produced in *E. coli* or eukaryotic expression systems, enabling biochemical and structural studies to dissect its mechanisms. Its recombinant form is pivotal for investigating RNA-protein interactions, helicase activity, and potential therapeutic targeting in disease contexts. Structural analyses, such as cryo-EM and X-ray crystallography, have advanced understanding of its functional domains and interaction networks.
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