| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX24 |
| Uniprot No | Q9GZR7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-804aa |
| 氨基酸序列 | MKLKDTKSRPKQSSCGKFQTKGIKVVGKWKEVKIDPNMFADGQMDDLVCFEELTDYQLVSPAKNPSSLFSKEAPKRKAQAVSEEEEEEEGKSSSPKKKIKLKKSKNVATEGTSTQKEFEVKDPELEAQGDDMVCDDPEAGEMTSENLVQTAPKKKKNKGKKGLEPSQSTAAIRDKLDILGAAETGSGKTLAFAIPMIHAVLQWQKRNAAPPPSNTEAPPGETRTEAGAETRSPGKAEAESDALPDDTVIESEALPSDIAAEARAKTGGTVSDQALLFGDDDAGEGPSSLIREKPVPKQNENEEENLDKEQTGNLKQELDDKSATCKAYPKRPLLGLVLTPTRELAVQVKQHIDAVARFTGIKTAILVGGMSTQKQQRMLNRRPEIVVATPGRLWELIKEKHYHLRNLRQLRCLVVDEADRMVEKGHFAELSQLLEMLNDSQYNPKRQTLVFSATLTLVHQAPARILHKKHTKKMDKTAKLDLLMQKIGMRGKPKVIDLTRNEATVETLTETKIHCETDEKDFYLYYFLMQYPGRSLVFANSISCIKRLSGLLKVLDIMPLTLHACMHQKQRLRNLEQFARLEDCVLLATDVAARGLDIPKVQHVIHYQVPRTSEIYVHRSGRTARATNEGLSLMLIGPEDVINFKKIYKTLKKDEDIPLFPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKEPQPEQPQPSTSAN |
| 分子量 | 116.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于真实研究领域的参考文献框架(注意：DDX24蛋白的具体重组研究文献可能较少，以下为模拟示例)：
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1. **"Functional characterization of recombinant human DDX24 protein in RNA splicing"**
*Author: Li X et al.*
*摘要*：本研究在大肠杆菌中表达了重组人DDX24蛋白，发现其通过ATP依赖的解旋酶活性参与pre-mRNA剪接调控，并揭示了其在癌症细胞中的异常表达。
2. **"Crystal structure and ATPase activity of the DEAD-box protein DDX24"**
*Author: Smith J et al.*
*摘要*：解析了重组人DDX24蛋白的晶体结构，阐明了其ATP结合域的关键残基，并通过突变实验验证了其与RNA结合能力的关联。
3. **"DDX24 interacts with viral RNA to inhibit influenza virus replication"**
*Author: Chen R et al.*
*摘要*：利用重组DDX24蛋白发现其通过与流感病毒RNA结合抑制病毒复制，为抗病毒药物开发提供了新靶点。
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**注意事项**：
1. 上述文献为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索关键词(如"recombinant human DDX24" "DEAD-box protein DDX24")。
2. DDX24的研究较少，可扩大检索范围至DEAD-box家族蛋白的功能共性。
3. 实验方法类文献可尝试关键词"DDX24 purification"或"DDX24 expression"。
如需具体文献协助，建议进一步提供研究背景(如病毒机制或癌症方向)。
Recombinant human DDX24 protein is a genetically engineered form of the DDX24 protein, encoded by the DDX24 gene located on human chromosome 14. As a member of the DEAD-box RNA helicase family, DDX24 contains conserved motifs (like the DEAD sequence) critical for ATP-dependent RNA unwinding and remodeling. It plays multifaceted roles in RNA metabolism, including RNA processing, folding, transport, and ribosome biogenesis, and is implicated in viral defense mechanisms by modulating host-cell RNA interactions.
Studies suggest DDX24 participates in cell cycle regulation, proliferation, and stress responses. Dysregulation of DDX24 expression has been observed in cancers, neurodegenerative disorders, and viral infections, highlighting its potential as a therapeutic target or biomarker. Recombinant DDX24 is typically produced in bacterial or eukaryotic expression systems, purified via affinity chromatography (e.g., His-tag), and used to study enzymatic activity, protein-RNA interactions, and structural characteristics. It enables in vitro exploration of DDX24's role in RNA-driven pathways, mechanisms of disease progression, and drug candidate screening. Despite advancements, its precise molecular interactions and pathophysiological relevance remain under active investigation, necessitating further functional studies to elucidate its regulatory networks.
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