| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX25 |
| Uniprot No | Q9UHL0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MASLLWGGDA GAAESERLNS HFSNLSQPRK NLWGIKSTAV RNIDGSINNI NEDDEEDVVD LAANSLLNKL IHQSLVESSH RVEVLQKDPS SPLYSVKTFE ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT AAFVLAMLSR VNALELFPQC LCLAPTYELA LQTGRVVEQM GKFCVDVQVM YAIRGNRIPR GTDITKQIII GTPGTVLDWC FKLKLIDLTK IRVFVLDEAD VMIDTQGFSD HSIRIQRALP SECQMLLFSA TFEDSVWHFA ERIIPDPNVI KLRKEELTLN NIRQYYVLCE HRKDKYQALC NIYGSITIGQ AIIFCQTRRN AKWLTVEMIQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR GIDVKQVTIV VNFDLPVKQG EEPDYETYLH RIGRTGRFGK KGLAFNMIEV DELPSLMKIQ DHFNSSIKQL NAEDMDEIEK IDY |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX25蛋白的模拟参考文献示例，涵盖不同研究方向：
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1. **标题**：*Recombinant Human DDX25 Facilitates RNA Unwinding in Spermatogenesis*
**作者**：Chen L., et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化重组人DDX25蛋白，发现其在体外具有ATP依赖的RNA解旋酶活性。实验表明，DDX25通过与生殖细胞特异性RNA结合，调控精子形成过程中的mRNA翻译，提示其在男性生殖中的关键作用。
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2. **标题**：*Structural Insights into the ATPase Activity of DDX25 via Cryo-EM Analysis*
**作者**：Tanaka K., et al.
**摘要**：通过冷冻电镜解析重组人DDX25蛋白的ATP结合结构域三维结构，揭示其与RNA结合的分子机制。研究发现突变关键氨基酸残基会显著降低其ATP水解活性，为靶向DDX25的疾病治疗提供结构基础。
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3. **标题**：*DDX25 Promotes Viral RNA Release in Host Cells: A Study Using Recombinant Protein*
**作者**：Wang Y., et al.
**摘要**：通过体外重构实验，证明重组人DDX25蛋白能够结合多种病毒RNA并促进其从核衣壳中释放。研究表明，干扰DDX25表达可抑制病毒复制，提示其作为抗病毒治疗潜在靶点。
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4. **标题**：*Functional Interaction Between DDX25 and p53 in Cancer Cell Apoptosis*
**作者**：Gupta R., et al.
**摘要**：利用重组人DDX25蛋白进行蛋白质互作实验，发现其与肿瘤抑制蛋白p53直接结合，并增强p53介导的凋亡相关基因转录。此相互作用在乳腺癌细胞系中显著影响化疗敏感性。
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**注**：以上文献为虚构示例，旨在模拟可能的真实研究方向和内容。实际写作中需替换为真实存在的参考文献。
**Background of Recombinant Human DDX25 Protein**
DDX25 (DEAD-box helicase 25), also known as GRTH (Gonadotropin-Regulated Testicular RNA Helicase), is a member of the DEAD-box RNA helicase family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. These ATP-dependent enzymes play critical roles in RNA metabolism, including unwinding RNA secondary structures, splicing, transport, and translational regulation. DDX25 is predominantly expressed in testicular germ cells and is hormonally regulated by gonadotropins, underscoring its functional significance in spermatogenesis. Studies suggest DDX25 participates in post-transcriptional regulation of mRNAs essential for germ cell development, particularly in late-stage spermatogenesis, by binding and stabilizing target transcripts or facilitating their translation.
Recombinant human DDX25 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. This recombinant protein retains conserved helicase domains and ATPase activity, enabling *in vitro* studies on its RNA-binding and unwinding mechanisms. Research applications include elucidating DDX25's role in male fertility, exploring its interactions with reproductive hormones, and investigating mutations linked to infertility. Furthermore, it serves as a tool for developing therapeutic strategies for reproductive disorders or as a biomarker for male fertility assessment. Its study bridges molecular biology, reproductive health, and genetic engineering.
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