| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX41 |
| Uniprot No | Q9UJV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 氨基酸序列 | MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGIHHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKREGPYGLIICPSRELARQTHGILEYYCRLLQEDSSPLLRCALCIGGMSVKEQMETIRHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEGDIRTIFSYFKGQRQTLLFSATMPKKIQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKTPPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATDVASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPPVLQVLHCGDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDYLAHSSMDF |
| 分子量 | 96.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX41蛋白的3篇文献示例(注：部分信息为模拟简化，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*DDX41 Recognizes Bacterial Secondary Messengers to Activate Immune Response*
**作者**：Zhang, Z. et al.
**摘要**：研究揭示了重组人DDX41蛋白作为先天免疫传感器，通过识别细菌环状二核苷酸(如c-di-AMP)激活STING信号通路，进而诱导I型干扰素产生的分子机制。
2. **文献名称**：*Germline Mutations in DDX41 Predispose to Myeloid Neoplasms*
**作者**：Jiang, Y. et al.
**摘要**：文章发现DDX41基因的胚系突变与家族性髓系肿瘤(如白血病)密切相关，并通过重组蛋白功能实验证明突变导致RNA解旋酶活性丧失，破坏DNA损伤修复和RNA代谢。
3. **文献名称**：*Structural Insights into the Helicase Activity of Human DDX41 Protein*
**作者**：Chen, X. et al.
**摘要**：通过解析重组人DDX41蛋白的晶体结构，揭示了其ATP依赖的RNA解旋酶活性核心域及关键功能位点，为靶向DDX41的药物设计提供了结构基础。
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如需具体文献，建议使用PubMed或Web of Science搜索关键词“recombinant DDX41”或“DDX41 function”获取最新研究。
Human DDX41 is a member of the DEAD-box family of ATP-dependent RNA helicases, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs crucial for RNA binding and unwinding. This protein plays diverse roles in RNA metabolism, including splicing, translation, and ribosome biogenesis. Notably, DDX41 has gained attention for its dual involvement in innate immune signaling and cancer pathogenesis. It acts as a cytosolic DNA sensor, facilitating the activation of the cGAS-STING pathway to initiate type I interferon responses against viral or bacterial DNA.
Germline mutations in DDX41 are linked to familial myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), while somatic mutations are observed in sporadic hematologic malignancies. These mutations often impair its helicase activity or disrupt protein stability, leading to dysregulated immune signaling or genomic instability. Structurally, DDX41 contains a helicase core domain, a nuclear localization signal, and a C-terminal domain involved in protein-protein interactions.
Recombinant human DDX41 protein is produced in expression systems (e.g., E. coli or mammalian cells) for functional studies, enabling exploration of its enzymatic properties, DNA/RNA binding affinity, and interaction with immune regulators. Its recombinant form is essential for developing targeted therapies, such as small-molecule inhibitors for hematologic cancers, and understanding its role in autoimmune and infectious diseases. Research on DDX41 bridges immunology, oncology, and molecular biology, highlighting its therapeutic and diagnostic potential.
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