| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX43 |
| Uniprot No | Q9NXZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 氨基酸序列 | MSHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEDVAAGHEELPLCFALKSHFVGAVIGRGGSKIKNIQSTTNTTIQIIQEQPESLVKIFGSKAMQTKAKAVIDNFVKKLEENYNSECGIDTAFQPSVGKDGSTDNNVVAGDRPLIDWDQIREEGLKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEKRPIPNPTCTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFKAHQQKREMERKMERPQGRPKKFH |
| 分子量 | 99.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDX43蛋白的3篇代表性文献的示例(文献信息为模拟虚构，仅供参考格式和内容方向)：
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1. **文献名称**: *DDX43 promotes tumor progression via the PI3K/AKT pathway in hepatocellular carcinoma*
**作者**: Li Y, et al.
**摘要**: 本研究报道了重组人DDX43蛋白在肝癌细胞中的促癌作用，通过激活PI3K/AKT信号通路促进细胞增殖和迁移，并证明其表达水平与肝癌患者预后呈负相关。
2. **文献名称**: *Recombinant expression and structural characterization of human DDX43 helicase domain*
**作者**: Smith J, et al.
**摘要**: 研究者通过大肠杆菌系统成功表达并纯化了重组人DDX43蛋白的ATP酶/解旋酶结构域，利用X射线晶体学解析其三维结构，揭示了其RNA结合的关键氨基酸残基。
3. **文献名称**: *DDX43 as a potential biomarker for early detection of colorectal cancer*
**作者**: Wang X, et al.
**摘要**: 该研究通过检测血清中重组DDX43蛋白的浓度，发现其在结直肠癌早期患者中显著升高，表明DDX43可能作为液体活检的潜在生物标志物。
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**注意**：以上文献为示例，建议通过PubMed、Google Scholar或Web of Science等平台，以“DDX43 recombinant protein”或“DDX43 cancer”等关键词检索真实文献。
Recombinant human DDX43 protein, a member of the DEAD-box RNA helicase family, is engineered to study the biological roles of this conserved ATP-dependent RNA helicase. DDX43 (also known as HAGE) contains the characteristic DEAD motif (Asp-Glu-Ala-Asp) and exhibits RNA-binding and unwinding activities, implicating it in transcriptional regulation, RNA metabolism, and epigenetic remodeling. While its physiological functions remain partially defined, DDX43 shows restricted expression in germline cells but is aberrantly overexpressed in various cancers, including melanoma, leukemia, and carcinomas. This ectopic expression correlates with tumor progression, metastasis, and therapy resistance, positioning DDX43 as both a potential oncogenic driver and a cancer-testis antigen target for immunotherapy.
The recombinant protein, produced via heterologous expression systems like E. coli or mammalian cells, enables functional characterization of DDX43's enzymatic properties and interactions with nucleic acids or partner proteins. Researchers employ it to dissect its role in cancer pathogenesis, including cell proliferation, apoptosis evasion, and genomic instability. Structural analyses using recombinant DDX43 aim to guide therapeutic inhibitor design. Additionally, its immunogenic properties are explored for cancer vaccine development or biomarker discovery. Challenges in production include maintaining post-translational modifications critical for activity, necessitating optimization of expression platforms. Ongoing studies seek to clarify DDX43's dual roles in normal gametogenesis and malignant transformation, bridging fundamental biology with clinical applications. (Word count: 247)
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