| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX4 |
| Uniprot No | Q9NQI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690aa |
| 氨基酸序列 | MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGDNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNKIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD |
| 分子量 | 102.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人DDX4蛋白相关研究的模拟参考文献(基于该领域常见研究方向构建)：
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1. **文献名称**：Expression and functional characterization of recombinant human DDX4/VASA helicase in insect cells
**作者**：M. Tanaka et al.
**摘要**：研究利用杆状病毒-昆虫细胞系统重组表达人DDX4蛋白，并通过ATP酶活性实验证实其RNA解旋酶功能。发现DDX4 C端结构域对其结合piRNA复合物至关重要，为生殖细胞特异性RNA调控机制提供依据。
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2. **文献名称**：Structural insights into human DDX4 protein by cryo-EM reveal nucleotide-dependent conformational changes
**作者**：J. Chen & R. Zhang
**摘要**：采用冷冻电镜解析重组人DDX4蛋白在结合ATP/ADP不同状态下的三维结构，揭示其N端结构域的柔性调控机制，提出该蛋白通过构象变化推动RNA解链的工作模型。
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3. **文献名称**：DDX4 knockdown impairs human induced pluripotent stem cell differentiation into germline lineage
**作者**：K. Lee et al.
**摘要**：通过siRNA沉默实验发现重组DDX4蛋白在hiPSC向原始生殖细胞(PGC)分化过程中调控关键基因(如NANOS3)的mRNA稳定性，证明其对人类生殖细胞发育的功能必要性。
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**领域知识补充**：
人DDX4(DEAD-box helicase 4)是生殖细胞特异性表达的RNA解旋酶，高度保守的DEAD结构域赋予其ATP依赖性RNA结合与重塑功能。现有研究多聚焦于：
1. 重组表达体系的优化(原核/真核系统)
2. 通过基因编辑技术(如CRISPR)探索其在配子发生中的调控网络
3. 结构生物学解析其与RNA/蛋白复合物的互作机制
可关注期刊：*Development*, *Nucleic Acids Research*, *Cell Reports*等。
Recombinant human DDX4 (also known as VASA) is a helicase protein encoded by the DDX4 gene, located on chromosome 5 in humans. A member of the DEAD-box RNA helicase family, it is primarily expressed in germline cells and plays critical roles in gametogenesis, including germ cell development, meiotic progression, and RNA metabolism. DDX4 is crucial for the formation and function of the nuage, a cytoplasmic structure involved in piRNA biosynthesis, which safeguards genome integrity by silencing transposable elements during embryogenesis.
The recombinant form is produced using expression systems like E. coli or mammalian cell cultures, enabling studies on its biochemical properties and interactions. Structurally, it contains conserved DEAD (Asp-Glu-Ala-Asp) and helicase domains responsible for ATP-dependent RNA unwinding. DDX4’s dynamic oligomerization and RNA-binding activity are essential for its role in translational regulation and mRNA stability in germ cells.
Research on recombinant DDX4 has advanced understanding of infertility, germ cell tumors, and developmental disorders linked to DDX4 mutations. It also serves as a biomarker for germ cell-derived cancers and aids in tracking in vitro-derived germ cells in regenerative medicine. Its conserved function across species makes it a model for studying evolutionary aspects of reproduction.
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